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Gene profiles of HUVECs in different phosphate concentration

ABSTRACT: Hyperphosphatemia is an independent risk factor for cardiovascular mortality in chronic kidney disease. High inorganic phosphorus can induce endothelial cell apoptosis, but the exact mechanism is not fully understood. This study addresses this knowledge gap.Microarray analysis was used to identify differentially expressed gene profiles in human umbilical vein endothelial cells (HUVECs) in high phosphate (3.0 mM) normal phosphate (1.0 mM) medium and low phosphate( 0.5mM). Differential gene expression in HUVECs seeded in high phosphate (3.0 mM ) medium ,normal phosphate ( 1.0 mM ) medium and simulated hypophosphatemia (0.5mM) were identified using Affymetrix GeneChip Human Gene 1.0 ST arrays ( Affymetrix ). Three chips were used to minimize the random error in each group.

ORGANISM(S): Homo sapiens

SUBMITTER: Ling Qin

PROVIDER: E-GEOD-60937 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene profiles of HUVECs in different phosphate concentration

Project description:Hyperphosphatemia is an independent risk factor for cardiovascular mortality in chronic kidney disease. High inorganic phosphorus can induce endothelial cell apoptosis, but the exact mechanism is not fully understood. This study addresses this knowledge gap.Microarray analysis was used to identify differentially expressed gene profiles in human umbilical vein endothelial cells (HUVECs) in high phosphate (3.0 mM) normal phosphate (1.0 mM) medium and low phosphate( 0.5mM).

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Project description:S. Typhimurium SL1344 parental strain and isogenic ΔrpoS, ΔrelAΔspoT and ΔrelAΔspoTΔrpoS strains were grown to OD600=1.0, OD600=2.3, OD600=3.0 and OD600=4.2 in Luria-Bertani medium.

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Transcriptomic profiles of S. Typhimurium SL1344 parent strain and ΔdksA and ΔrelAΔspoTΔdksA mutant strains grown to various optical densities during late-log and stationary phase in LB aerobic batch culture

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Metformin induces chromosome reorganization and changes in gene expression in normal human fibroblasts

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Delayed phosphate starvation response in cells with abundant high-affinity transporters

Project description:Wild-type cells and cells with abundant high-affinity transporters (PHO84C) were transferred from rich medium (SD) to medium containing 0.5mM phosphate Cells were grown to logarithmic phase in SC medium (OD=0.5), washed and then incubated in SC medium with intermediate phosphate level (0.5mM, initial OD=0.05). During the experiment (0, 1, 5, 8 and 23 hours) cells were harvested, pelleted and frozen for further analysis. Total RNA was extracted using MasterPure™fication Kit (Epicentre). The samples were amplified, labeled, hybridized to yeast dual color expression microarrays and scanned, all using standard Agilent protocols, reagents, and instruments. The scanned images were analyzed using SpotReader software (Niles Scientific).

2011-09-14 | E-GEOD-32067 | biostudies-arrayexpress

Stimulation of human umbilical venous endothelial cells (HUVECs) with bone morphogenetic protein 4 (BMP4).

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