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Development of microRNA (miRNA) expression signatures for human glioma-initiating cells (GICs)

ABSTRACT: To identify a novel miRNA that is aberrantly expressed in GICs, we analyzed differences in miRNA expression between the human GICs and glioma cell lines and neural stem cells by miRNA microarrays. We examined the miRNA expression profiles of five human GICs that were obtained from human glioma samples and two human glioma cell lines, U87 and U251, and NSC (neural stem cells) as a control.

ORGANISM(S): Homo sapiens

SUBMITTER: Daisuke Yamashita

PROVIDER: E-GEOD-61079 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miR340 suppresses the stem-like cell function of glioma-initiating cells by targeting tissue plasminogen activator.

Yamashita Daisuke D Kondo Toru T Ohue Shiro S Takahashi Hisaaki H Ishikawa Madoka M Matoba Ryo R Suehiro Satoshi S Kohno Shohei S Harada Hironobu H Tanaka Junya J Ohnishi Takanori T

Cancer research 20150127 6

Glioma-initiating cells (GIC) have stem-like cell properties thought to be sufficient for recurrence, progression, and drug resistance in glioblastomas. In the present study, we defined miRNA (miR)-340 as a differentially expressed miRNA in human GICs that inhibit GIC-mediated tumorigenesis. Furthermore, we defined tissue plasminogen activator (PLAT) as a critical direct target of miR340 for inhibition. Among miRNAs screened, we found that miR340 expression was decreased in all human GICs and in ...[more]

PMID: 25627976

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