Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

55 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide analysis of whole transcriptome in E(z) temperature sensitive cells [RNA-Seq]

ABSTRACT: PcG protein complex PRC2 is a methyltransferase specific for histone H3 lysine27, and H3K27me3 is essential for stable transcription silencing. Less well known but quantitatively much more important is the genome-wide role of PRC2 that dimethylates ~70% of total H3K27. Here we show that H3K27me2 occurs in inverse proportion to transcriptional activity in genes and intergenic regions and its loss results in global transcriptional derepression proportionally greatest in previously silent or weakly transcribed regions. H3K27me2 levels are controlled by opposing roaming activities of PRC2 and the H3K27 demethylase dUTX. Unexpectedly, we find an equally pervasive distribution of histone H2A ubiquitylated at lysine 118 (H2AK118ub), attributed to the RING1 subunit of PRC1-type complexes. Examination of global changes in transcription genome-wide when E(z) is inactivated by monitoring total RNA from E(z) temperature-sensitive cells at 25°C and 31°C in duplicate

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Hun-Goo Lee

PROVIDER: E-GEOD-61307 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Genome-wide analysis of histone marks in E(z) temperature sensitive cells [ChIP-seq]

Project description:PcG protein complex PRC2 is a methyltransferase specific for histone H3 lysine27, and H3K27me3 is essential for stable transcription silencing. Less well known but quantitatively much more important is the genome-wide role of PRC2 that dimethylates ~70% of total H3K27. Here we show that H3K27me2 occurs in inverse proportion to transcriptional activity in genes and intergenic regions and its loss results in global transcriptional derepression proportionally greatest in previously silent or weakly transcribed regions. H3K27me2 levels are controlled by opposing roaming activities of PRC2 and the H3K27 demethylase dUTX. Unexpectedly, we find an equally pervasive distribution of histone H2A ubiquitylated at lysine 118 (H2AK118ub), attributed to the RING1 subunit of PRC1-type complexes. Examination of global changes in five epigenetic marks when E(z) is inactivated in E(z) temperature-sensitive cells at 25°C and 31°C.

2015-05-14 | E-GEOD-64219 | biostudies-arrayexpress

Genome-wide analysis of epigenetic marks in E(z) temperature sensitive cells [ChIP-seq]

2015-05-14 | E-GEOD-61308 | biostudies-arrayexpress

Interplay between Nsd1 and PRC2 demarcates regions of H3K27me2 and H3K27me3

Project description:The core Polycomb Repressor Complex 2 (PRC2) is composed of Ezh1/2, Suz12, Eed and is responsible for mediating both H3K27me2 and H3K27me3. However, the mechanisms by which PRC2 demarcates these two repressive modifications in the genome are unknown. In a functional screen, we identified the H3K36 dimethyltransferase Nsd1 as a modulator of PRC2-mediated di- and trimethylation of H3K27. ChIP-Seq analysis following the depletion of Nsd1 revealed a global reduction in H3K36me2 and an increase in H3K27me3 at sites previously marked by H3K27me2. We show that the H3K36me2 at H3K27me2 marks co-occupy regions and provide evidence that the presence of H3K36me2 functions to restrict the spatial distribution of Polycomb mediated H3K27me3 domains.

2018-09-15 | GSE86925 | GEO

Interplay between Nsd1 and PRC2 demarcates regions of H3K27me2 and H3K27me3.

2018-03-29 | GSE107773 | GEO

Distribution of H3K27me2, dUTX, and histone H3 in Sg4 cells

2015-05-14 | E-GEOD-61261 | biostudies-arrayexpress

Distinct Roles of H3K27me2 and H3K27me3 States Unveiled By Fate Specification of Embryonic Stem Cells

Project description:The Polycomb Repressive Complex 2 (PRC2) induces methylation of lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have indeed different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESC) with a modified H3K27me2/H3K27me3 ratio. Our findings document a developmental dynamic control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESC. They also reveal that modifying the H3K27me2/H327me3 ratio is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes, along with influencing induction of the ESC ground state.

2016-09-01 | GSE85717 | GEO

Genome-wide distribution of Rad26 and Rad1-Rad10 in relation to Mediator and RNA polymerase II

Project description:ChIPseq of Rad26, Rad1 and Rad10 proteins indicate their presence on chromatin in absence of UV-stress. In addition, in kin28ts mutant context, their genomic profiles change suggesting the influence of both Mediator complex and PolII transcription

2022-06-17 | E-MTAB-10828 | biostudies-arrayexpress

Genome-wide analysis of histone marks in E(z) temperature sensitive cells [ChIP-seq]

2015-05-14 | GSE64219 | GEO

Genome-wide analysis of epigenetic marks in E(z) temperature sensitive cells [ChIP-seq]

2015-05-14 | GSE61308 | GEO

Genome-wide analysis of whole transcriptome in E(z) temperature sensitive cells [RNA-Seq]

2015-05-14 | GSE61307 | GEO