Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide distribution of Rad26 and Rad1-Rad10 in relation to Mediator and RNA polymerase II

ABSTRACT: ChIPseq of Rad26, Rad1 and Rad10 proteins indicate their presence on chromatin in absence of UV-stress. In addition, in kin28ts mutant context, their genomic profiles change suggesting the influence of both Mediator complex and PolII transcription

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Saccharomyces cerevisiae S288C

SUBMITTER: Cyril DENBY WILKES

PROVIDER: E-MTAB-10828 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Functional interplay between Mediator and RSC chromatin remodeling complex in nucleosome-depleted region maintenance at promoters

Project description:ChIPseq of Mediator and RSC in wildtype or mutant context reveal their co-occupancy genome-wide. In addition MNAse-seq experiments in the same contexts shows the specific impact of the mutations on nucleosome positioning/remodelling.

2023-05-11 | E-MTAB-12198 | biostudies-arrayexpress

The genome-scale DNA-binding of FadR transcription factor in Sulfolobus acidocaldarius

Project description:This is ChIPseq result of FadR, which is the only TetR family regulator presented in Sulfolobus acidocaldarius. The aim of the study is to gain insights into the function of TetR regulator by analyzing its whole genome binding sites.

2017-12-30 | E-MTAB-6328 | biostudies-arrayexpress

Genome-wide analysis of histone marks in E(z) temperature sensitive cells [ChIP-seq]

Project description:PcG protein complex PRC2 is a methyltransferase specific for histone H3 lysine27, and H3K27me3 is essential for stable transcription silencing. Less well known but quantitatively much more important is the genome-wide role of PRC2 that dimethylates ~70% of total H3K27. Here we show that H3K27me2 occurs in inverse proportion to transcriptional activity in genes and intergenic regions and its loss results in global transcriptional derepression proportionally greatest in previously silent or weakly transcribed regions. H3K27me2 levels are controlled by opposing roaming activities of PRC2 and the H3K27 demethylase dUTX. Unexpectedly, we find an equally pervasive distribution of histone H2A ubiquitylated at lysine 118 (H2AK118ub), attributed to the RING1 subunit of PRC1-type complexes. Examination of global changes in five epigenetic marks when E(z) is inactivated in E(z) temperature-sensitive cells at 25°C and 31°C.

2015-05-14 | E-GEOD-64219 | biostudies-arrayexpress

Genome-wide analysis of epigenetic marks in E(z) temperature sensitive cells [ChIP-seq]

2015-05-14 | E-GEOD-61308 | biostudies-arrayexpress

Genome-wide analysis of whole transcriptome in E(z) temperature sensitive cells [RNA-Seq]

Project description:PcG protein complex PRC2 is a methyltransferase specific for histone H3 lysine27, and H3K27me3 is essential for stable transcription silencing. Less well known but quantitatively much more important is the genome-wide role of PRC2 that dimethylates ~70% of total H3K27. Here we show that H3K27me2 occurs in inverse proportion to transcriptional activity in genes and intergenic regions and its loss results in global transcriptional derepression proportionally greatest in previously silent or weakly transcribed regions. H3K27me2 levels are controlled by opposing roaming activities of PRC2 and the H3K27 demethylase dUTX. Unexpectedly, we find an equally pervasive distribution of histone H2A ubiquitylated at lysine 118 (H2AK118ub), attributed to the RING1 subunit of PRC1-type complexes. Examination of global changes in transcription genome-wide when E(z) is inactivated by monitoring total RNA from E(z) temperature-sensitive cells at 25°C and 31°C in duplicate

2015-05-14 | E-GEOD-61307 | biostudies-arrayexpress

Chromatin isolation by RNA-purification sequencing assay (ChIRP-seq) for RAIN in papillary thyroid cancer cell line TPC1

Project description:Characterization of the genomic distribution of RAIN in thyroid cancer. RAIN was pulled-down using two sets of 3’ BiotinTEG probes (EVEN and ODD). Subsequently, the DNA bound by RAIN was sequenced to assess its biding genome wide.

2024-09-13 | E-MTAB-13776 | biostudies-arrayexpress

ChIP-seq for Meis on mouse branchial arches at E11.5.

Project description:Meis ChIP-seq on mouse first branchial arch (BA1) and posterior branchial arches connected to outflow tract of the heart (PBA/OFT) at embryonic day (E) 11.5.

2020-01-06 | E-MTAB-7767 | biostudies-arrayexpress

Sequencing of synovial tissue fibroblasts from Rheumatoid Arthritis patients that were co-cultured with inflammatory macrophages.

Project description:The role of synovial tissue fibroblasts and macrophages interactions in driving chronic inflammation and resolution of arthritis is unknown. In this project, we used bulk RNAseq to investigate the impact of different phenotypes of macrophages on activation of synovial fibroblasts.

2020-04-18 | E-MTAB-8316 | biostudies-arrayexpress

scRNA-seq of Gallus gallus embryo xenografts to investigate how the embryonic node functions as an instructive stem cell niche

Project description:During development, the amniote organizer, Hensen’s node, contributes cells to the developing axis in head-to-tail direction. However, some cells remain resident in the node and it has been suggested that these resident cells are stem cells. This study aimed to characterise single resident cells and their environment within the node. Using the chick as an amniote model, we generated transcriptomes of single resident cells (scRNA-seq of GFP cells that remained resident till stage-HH8 following a GFP-donor homotopic transplant into a non-GFP host) and of six node sub-regions (bulk RNA-seq of GFP-transgenic stage-HH8 node, but otherwise un-manipulated embryos). We also obtained transcriptomes of single cells with resident behaviour that would normally never enter the node, but were made to do so (scRNA-seq of cells that remained resident till HH8 following a GFP-donor heterotopic/heterochronic transplant into a non-GFP host).

2022-02-02 | E-MTAB-11216 | biostudies-arrayexpress

The embryonic node functions as an instructive stem cell niche (scRNA-seq)

2022-02-02 | E-MTAB-9116 | biostudies-arrayexpress