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Transcriptome of Arabidopsis thaliana ceh1 mutant

ABSTRACT: Here we present the transcriptomic profile of mutant plants designated as ceh1 (constitutively expressing HPL). CEH1 encodes 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase (HDS), the enzyme controlling the bottleneck step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the plastids. Mutation of this enzyme in ceh1 mutant led to accumulation of high levels of the stress specific signaling metabolite 2c-methyl-D-erythritol 2,4-cylclodiphosphate (MEcPP), and consequently constitutive activation of a selected otherwise stress responsive genes. This data identifies the ensemble of stress responsive genes whose expression is regulated by the MEcPP signaling cascade. About 20 two-week-old plants grown on 1/2 MS medium were collected to extract total RNA. Transcriptome of parent line and ceh1 mutant were compared.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Katayoon Dehesh 

PROVIDER: E-GEOD-61675 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Plastid-produced interorgannellar stress signal MEcPP potentiates induction of the unfolded protein response in endoplasmic reticulum.

Walley Justin J   Xiao Yanmei Y   Wang Jin-Zheng JZ   Baidoo Edward E EE   Keasling Jay D JD   Shen Zhouxin Z   Briggs Steven P SP   Dehesh Katayoon K  

Proceedings of the National Academy of Sciences of the United States of America 20150428 19

Cellular homeostasis in response to internal and external stimuli requires a tightly coordinated interorgannellar communication network. We recently identified methylerythritol cyclodiphosphate (MEcPP) as a novel stress-specific retrograde signaling metabolite that accumulates in response to environmental perturbations to relay information from plastids to the nucleus. We now demonstrate, using a combination of transcriptome and proteome profiling approaches, that mutant plants (ceh1) with high  ...[more]

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