Proteome of Arabidopsis thaliana eds16, ceh1, and eds16 ceh1 mutants
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)
SUBMITTER: Katayoon Dehesh
PROVIDER: MSV000078868 | MassIVE |
REPOSITORIES: MassIVE
Similar Datasets
Project description:Here we present the transcriptomic profile of mutant plants designated as ceh1 (constitutively expressing HPL). CEH1 encodes 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase (HDS), the enzyme controlling the bottleneck step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the plastids. Mutation of this enzyme in ceh1 mutant led to accumulation of high levels of the stress specific signaling metabolite 2c-methyl-D-erythritol 2,4-cylclodiphosphate (MEcPP), and consequently constitutive activation of a selected otherwise stress responsive genes. This data identifies the ensemble of stress responsive genes whose expression is regulated by the MEcPP signaling cascade.
2014-09-24 | GSE61675 | GEO
Project description:Here we present the transcriptomic profile of mutant plants designated as ceh1 (constitutively expressing HPL). CEH1 encodes 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase (HDS), the enzyme controlling the bottleneck step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the plastids. Mutation of this enzyme in ceh1 mutant led to accumulation of high levels of the stress specific signaling metabolite 2c-methyl-D-erythritol 2,4-cylclodiphosphate (MEcPP), and consequently constitutive activation of a selected otherwise stress responsive genes. This data identifies the ensemble of stress responsive genes whose expression is regulated by the MEcPP signaling cascade. About 20 two-week-old plants grown on 1/2 MS medium were collected to extract total RNA. Transcriptome of parent line and ceh1 mutant were compared.
2014-09-24 | E-GEOD-61675 | biostudies-arrayexpress
Project description:In this study we show the comparative transcriptome of constitutive subtilisin3 (csb3) plants, an Arabidopsis mutant showing strikingly enhanced resistance to biotrophic pathogens. CSB3 encodes a 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase, the enzyme controlling the penultimate step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the chloroplast. It has been proposed that CSB3 represents a point of metabolic convergence modulating the magnitude of SA-mediated disease resistance to biotrophic pathogens. We show that csb3 have increased expression of a set of genes encoding defense-related proteins and enzymes, which includes two subtilisins. In essence our results substantiates an important role of these two subtilases in the activation of defense-related signaling pathways against biotrophic pathogens.
2014-04-25 | GSE35507 | GEO
Project description:In this study we show the comparative transcriptome of constitutive subtilisin3 (csb3) plants, an Arabidopsis mutant showing strikingly enhanced resistance to biotrophic pathogens. CSB3 encodes a 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase, the enzyme controlling the penultimate step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the chloroplast. It has been proposed that CSB3 represents a point of metabolic convergence modulating the magnitude of SA-mediated disease resistance to biotrophic pathogens. We show that csb3 have increased expression of a set of genes encoding defense-related proteins and enzymes, which includes two subtilisins. In essence our results substantiates an important role of these two subtilases in the activation of defense-related signaling pathways against biotrophic pathogens. 5 five-week-old plants were collected, frozen in liquid nitrogen and stored at -80ºC. Samples from Col-0 and csb3 were compared.
2014-04-25 | E-GEOD-35507 | biostudies-arrayexpress
Project description:TIFY is a large plant-specific transcription factor gene family. A subgroup of TIFY genes named JAZ (Jasmonate-ZIM domain) has been identified as repressors of jasmonate (JA)-regulated transcription in Arabidopsis and other plants. JA signaling is involved in many aspects of plant growth/development and in the defense responses to biotic and abiotic stresses. Here we identified the TIFY genes (designated as PvTIFY) from the legume common bean (Phaseolus vulgaris) and functionally characterized PvTIFY10C as a transcriptional regulator. Twenty-three genes from the PvTIFY gene family were identified through whole genome sequence analysis. Most of these were induced upon methyl-JA elicitation. We selected PvTIFY10C as a representative JA-responsive PvTIFY gene for further functional analysis. Transcriptome analysis via microarray hybridization using the designed Bean Custom Array 90K was performed in transgenic roots of composite plants with modulated -RNAi-silencing or over-expression- PvTIFY10C gene expression. Data were interpreted using Mapman adapted to common bean. Microarray differential gene expression data were validated by real-time qRT-PCR expression analysis. Comparative global gene expression analysis revealed opposite regulatory changes in processes such as RNA and protein regulation, stress response and metabolism in silenced vs. over-expressing roots. These data point to transcript reprogramming -mainly repression- orchestrated by PvTIFY10C. In addition we found that several PvTIFY genes as well as genes from the JA biosynthetic pathway responded to P-deficiency. Relevant P-responsive genes that participate in carbon metabolic pathways, cell wall synthesis, lipid metabolism, transport, DNA, RNA and protein regulation, signaling, were oppositely-regulated in control vs. PvTIFY10C silenced roots. These data indicate that PvTIFY10C regulates, directly or indirectly, gene expression of some P-responsive genes something that could be mediated by JA-signaling. Our work contributed to the functional characterization of PvTIFY transcriptional regulators in common bean, an agronomically important legume. Members from the large PvTIFY gene family are important global transcriptional regulators that could participate as repressors of the JA signaling pathway. In addition we propose that the JA-signaling pathway that involves PvTIFY genes might play a role in regulating the plant response / adaptation to P-starvation.
2013-02-27 | E-GEOD-40935 | biostudies-arrayexpress
Project description:Gmdnj1-knockout mutants had diminished growth in normal conditions, and when under heat stress, exhibited more severe browning, reduced chlorophyll contents, higher reactive oxygen species (ROS) contents, and higher induction of heat stress-responsive transcription factors and ROS-scavenging enzyme-encoding genes. Knocking out a major HSP40 may lead to the impairment of the protein refolding system, and in turn lead to a change in the aggregated protein profile. Therefore, the aggregated protein profiles of the Gmdnj1 mutant lines were compared against that of the wild type Williams 82 under both untreated and heat-treated conditions.
2021-09-09 | PXD019799 | Pride
Project description:Interventions: Experimental group:ß- hydroxy- ß- methyl butyrate;Control Group:No
Primary outcome(s): skeletal muscle index;tumor necrosis factor-like weak apoptosis-inducing factor;peroxisome proliferator-activated receptor coactivator 1-a;growth differentiation factor 15;grip strength;pace;leg circumference
Study Design: Parallel
| 2716507 | ecrin-mdr-crc
Project description:TIFY is a large plant-specific transcription factor gene family. A subgroup of TIFY genes named JAZ (Jasmonate-ZIM domain) has been identified as repressors of jasmonate (JA)-regulated transcription in Arabidopsis and other plants. JA signaling is involved in many aspects of plant growth/development and in the defense responses to biotic and abiotic stresses. Here we identified the TIFY genes (designated as PvTIFY) from the legume common bean (Phaseolus vulgaris) and functionally characterized PvTIFY10C as a transcriptional regulator. Twenty-three genes from the PvTIFY gene family were identified through whole genome sequence analysis. Most of these were induced upon methyl-JA elicitation. We selected PvTIFY10C as a representative JA-responsive PvTIFY gene for further functional analysis. Transcriptome analysis via microarray hybridization using the designed Bean Custom Array 90K was performed in transgenic roots of composite plants with modulated -RNAi-silencing or over-expression- PvTIFY10C gene expression. Data were interpreted using Mapman adapted to common bean. Microarray differential gene expression data were validated by real-time qRT-PCR expression analysis. Comparative global gene expression analysis revealed opposite regulatory changes in processes such as RNA and protein regulation, stress response and metabolism in silenced vs. over-expressing roots. These data point to transcript reprogramming -mainly repression- orchestrated by PvTIFY10C. In addition we found that several PvTIFY genes as well as genes from the JA biosynthetic pathway responded to P-deficiency. Relevant P-responsive genes that participate in carbon metabolic pathways, cell wall synthesis, lipid metabolism, transport, DNA, RNA and protein regulation, signaling, were oppositely-regulated in control vs. PvTIFY10C silenced roots. These data indicate that PvTIFY10C regulates, directly or indirectly, gene expression of some P-responsive genes something that could be mediated by JA-signaling.
2013-02-27 | GSE40935 | GEO
Project description:Abstract: A sensor histidine kinase of Synechococcus sp. strain PCC7942, designated nblS, was previously identified and shown to be critical for the acclimation of cells to high-light and nutrient limitation conditions and to influence the expression of a number of light-responsive genes. The nblS orthologue in Synechocystis sp. strain PCC6803 is designated dspA (also called hik33). We have generated a dspA null mutant and analyzed global gene expression in both the mutant and wild-type strains under high- and low-light conditions. The mutant is aberrant for the expression of many genes encoding proteins critical for photosynthesis, phosphate and carbon acquisition, and the amelioration of stress conditions. Furthermore, transcripts from a number of genes normally detected only during exposure of wild-type cells to high-light conditions become partially constitutive in the low-light-grown dspA mutant. Other genes for which transcripts decline upon exposure of wild-type cells to high light are already lower in the mutant during growth in low light. These results suggest that DspA may influence gene expression in both a positive and a negative manner and that the dspA mutant behaves as if it were experiencing stress conditions (e.g., high-light exposure) even when maintained at near-optimal growth conditions for wild-type cells. This is discussed with respect to the importance of DspA for regulating the responses of the cell to environmental cues. A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species. Keywords: strain_or_line_design
2006-01-19 | GSE4019 | GEO