Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1120962 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Here we present the transcriptomic profile of mutant plants designated as ceh1 (constitutively expressing HPL). CEH1 encodes 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase (HDS), the enzyme controlling the bottleneck step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the plastids. Mutation of this enzyme in ceh1 mutant led to accumulation of high levels of the stress specific signaling metabolite 2c-methyl-D-erythritol 2,4-cylclodiphosphate (MEcPP), and consequently constitutive activation of a selected otherwise stress responsive genes. This data identifies the ensemble of stress responsive genes whose expression is regulated by the MEcPP signaling cascade. About 20 two-week-old plants grown on 1/2 MS medium were collected to extract total RNA. Transcriptome of parent line and ceh1 mutant were compared.
2014-09-24 | E-GEOD-61675 | biostudies-arrayexpress
Project description:Here we present the transcriptomic profile of mutant plants designated as ceh1 (constitutively expressing HPL). CEH1 encodes 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase (HDS), the enzyme controlling the bottleneck step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the plastids. Mutation of this enzyme in ceh1 mutant led to accumulation of high levels of the stress specific signaling metabolite 2c-methyl-D-erythritol 2,4-cylclodiphosphate (MEcPP), and consequently constitutive activation of a selected otherwise stress responsive genes. This data identifies the ensemble of stress responsive genes whose expression is regulated by the MEcPP signaling cascade.
2014-09-24 | GSE61675 | GEO
Project description:Multiple Organellar RNA Editing Factor 2 (MORF2) was previously discovered to be involved in chloroplast RNA editing. However, the albino and unviable phenotype of the T-DNA insertion mutants, morf2-1 and morf2-2, seems to be inconsistent with many other viable chloroplast RNA editing mutants, suggesting that MORF2 has other biological functions beyond RNA editing.
2023-04-19 | GSE213405 | GEO
Project description:Here we present the proteomic profile of mutant plants designated as eds16 (enhanced disease susceptibility), ceh1 (constitutively expressing HPL) as well as the ceh1 eds16 double mutant. CEH1 encodes 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase (HDS), the enzyme controlling the bottleneck step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the plastids. Mutation of this enzyme in ceh1 mutant led to accumulation of high levels of the stress specific signaling metabolite 2c-methyl-D-erythritol 2,4-cylclodiphosphate (MEcPP), and consequently constitutive activation of a selected otherwise stress responsive genes. This data identifies the ensemble of stress responsive genes whose expression is regulated by the MEcPP signaling cascade.
| MSV000078868 | MassIVE
Project description:In this study we show the comparative transcriptome of constitutive subtilisin3 (csb3) plants, an Arabidopsis mutant showing strikingly enhanced resistance to biotrophic pathogens. CSB3 encodes a 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase, the enzyme controlling the penultimate step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the chloroplast. It has been proposed that CSB3 represents a point of metabolic convergence modulating the magnitude of SA-mediated disease resistance to biotrophic pathogens. We show that csb3 have increased expression of a set of genes encoding defense-related proteins and enzymes, which includes two subtilisins. In essence our results substantiates an important role of these two subtilases in the activation of defense-related signaling pathways against biotrophic pathogens. 5 five-week-old plants were collected, frozen in liquid nitrogen and stored at -80ºC. Samples from Col-0 and csb3 were compared.
2014-04-25 | E-GEOD-35507 | biostudies-arrayexpress
Project description:In this study we show the comparative transcriptome of constitutive subtilisin3 (csb3) plants, an Arabidopsis mutant showing strikingly enhanced resistance to biotrophic pathogens. CSB3 encodes a 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase, the enzyme controlling the penultimate step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the chloroplast. It has been proposed that CSB3 represents a point of metabolic convergence modulating the magnitude of SA-mediated disease resistance to biotrophic pathogens. We show that csb3 have increased expression of a set of genes encoding defense-related proteins and enzymes, which includes two subtilisins. In essence our results substantiates an important role of these two subtilases in the activation of defense-related signaling pathways against biotrophic pathogens.
2014-04-25 | GSE35507 | GEO
Project description:The RNA editing enzyme ADAR1 is a key regulatory of innate immune responses to RNA
| PRJNA266103 | ENA
Project description:Study of the role of the FLV/DOT4 protein in post-transcriptional regulation of chloroplast gene expression. DOT4 is a pentatricopeptide repeat protein targeted to the chloroplast which regulates the editing of the rpoC1 transcript The editing level of rpoC1 varies from one tissue to the other and because the main macroscopic phenotype of the flv/dot4 mutant are white leaf margins. We compared the leaf border to the leaf center of wild-type Col0 plants but also the leaf borders of col0 and flv/dot4 knock out mutants by sequencing total RNA depleted from rRNA to get a global view of gene expression (including post-transcrional modifications) of the 3 plant genomes: nucleus, chloroplast and mitochondria. mRNA seq on wild-type Col and FLV mutants knock out.
2016-02-12 | E-GEOD-71149 | biostudies-arrayexpress
Project description:Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3B) is a key molecular driver inducing mutations in multiple human cancer. A3B belongs to the APOBEC3 enzyme family, which consists seven closely related DNA deaminases that catalyse cytosine-to-uracil (C>U) editing of single-stranded DNA (ssDNA). Using a cell model that lacks base excision repair function, we sampled A3B editing sites in bulk. Analyses conducted on these A3B editing sites point to a function of A3B in editing DNA:RNA hybrid structure known as R-loops. In order to verify this result, we conducted strand-specific DNA:RNA immunoprecipitation sequencing (ssDRIP-seq; S9.6, KeraFAST, ENH001) on T-47D breast cancer cells.
2025-01-06 | GSE193229 | GEO
Project description:Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3B) is a key molecular driver inducing mutations in multiple human cancer. A3B is a member of the APOBEC3 enzyme family, which consists seven closely related DNA deaminases that catalyse cytosine-to-uracil (C>U) editing of single-stranded DNA (ssDNA). Using a cell model that lacks base excision repair function, we sampled A3B editing sites in bulk. Analyses conducted on these A3B editing sites point to a function of A3B in editing DNA:RNA hybrid structure known as R-loops. In order to verify this result, we conducted strand-specific DNA:RNA immunoprecipitation sequencing (ssDRIP-seq; S9.6, KeraFAST, ENH001) on T-47D breast cancer cells.
2025-01-06 | GSE193225 | GEO