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Unknown,Transcriptomics,Genomics,Proteomics

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Low dose of DNA-demethylating agents target colorectal cancer-initiating cells by activation of RIG1/MAVS signaling pathway

ABSTRACT: Genome wide DNA methylation profiling of 3 patient-derived colorectal cancer samples with different CIMP profiles. The Illumina Infinium 450k Human DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 450,000 CpGs Bisulphite converted DNA from the 3 samples were hybridised to the Illumina HumanMethylation450 BeadChip

ORGANISM(S): Homo sapiens

SUBMITTER: Daniel De Carvalho

PROVIDER: E-GEOD-62086 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA-Demethylating Agents Target Colorectal Cancer Cells by Inducing Viral Mimicry by Endogenous Transcripts.

Roulois David D Loo Yau Helen H Singhania Rajat R Wang Yadong Y Danesh Arnavaz A Shen Shu Yi SY Han Han H Liang Gangning G Jones Peter A PA Pugh Trevor J TJ O'Brien Catherine C De Carvalho Daniel D DD De Carvalho Daniel D DD

Cell 20150801 5

DNA-demethylating agents have shown clinical anti-tumor efficacy via an unknown mechanism of action. Using a combination of experimental and bioinformatics analyses in colorectal cancer cells, we demonstrate that low-dose 5-AZA-CdR targets colorectal cancer-initiating cells (CICs) by inducing viral mimicry. This is associated with induction of dsRNAs derived at least in part from endogenous retroviral elements, activation of the MDA5/MAVS RNA recognition pathway, and downstream activation of IRF ...[more]

PMID: 26317465

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