Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression of T47D-MTVL human breast cancer cells upon H1X knock-down


ABSTRACT: Gene expression of T47D-MTVL human breast cancer cells expressing Dox-inducible shRNAs against histone H1X Stable breast cancer-derived cell lines expressing an shRNA against the histone H1X isoforms in response to doxycycline (Dox) were grown for six days in the presence or absence of Dox, and serum-starved for the last 48h for synchronization in G1, in duplicate, and RNA extracted for microarray hybridization. Cell lines used: inducible shRNA against H1X, and random shRNA-expression vector as a control.

ORGANISM(S): Homo sapiens

SUBMITTER: Lauro Sumoy 

PROVIDER: E-GEOD-62766 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions.

Mayor Regina R   Izquierdo-Bouldstridge Andrea A   Millán-Ariño Lluís L   Bustillos Alberto A   Sampaio Cristina C   Luque Neus N   Jordan Albert A  

The Journal of biological chemistry 20150202 12


Unlike core histones, the linker histone H1 family is more evolutionarily diverse, and many organisms have multiple H1 variants or subtypes. In mammals, the H1 family includes seven somatic H1 variants; H1.1 to H1.5 are expressed in a replication-dependent manner, whereas H1.0 and H1X are replication-independent. Using ChIP-sequencing data and cell fractionation, we have compared the genomic distribution of H1.0 and H1X in human breast cancer cells, in which we previously observed differential d  ...[more]

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