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Gene expression patterns in responding versus non-responding AB1-HA tumors from mice during immunotherapy

ABSTRACT: Balb/c mice were inoculated subcutaneously with AB1-HA cells on both flanks on day 0. The mice were then treated with anti-CTLA-4 on day 5 or 6. 7 days later, one tumor was removed for gene expression studies, and the other was left in situ to monitor tumor growth and determine if the eventual outcome was progression or regression. The study design consisted of three experimental groups; (i) untreated mice; (ii) anti-CTLA-4 treated responders; (iii) anti-CTLA-4 treated nonresponders. There were 10 animals in each group.

ORGANISM(S): Mus musculus

SUBMITTER: Anthony Bosco

PROVIDER: E-GEOD-63557 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Network analysis of immunotherapy-induced regressing tumours identifies novel synergistic drug combinations.

Lesterhuis W Joost WJ Rinaldi Catherine C Jones Anya A Rozali Esdy N EN Dick Ian M IM Khong Andrea A Boon Louis L Robinson Bruce W BW Nowak Anna K AK Bosco Anthony A Lake Richard A RA

Scientific reports 20150721

Cancer immunotherapy has shown impressive results, but most patients do not respond. We hypothesized that the effector response in the tumour could be visualized as a complex network of interacting gene products and that by mapping this network we could predict effective pharmacological interventions. Here, we provide proof of concept for the validity of this approach in a murine mesothelioma model, which displays a dichotomous response to anti-CTLA4 immune checkpoint blockade. Network analysis ...[more]

PMID: 26193793

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