Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human right ventricular samples acquired during cardiac surgery

ABSTRACT: Right ventricular samples were serially acquired during surgical repair of ventricular septal defect. Expression profiling revealed three patterns of gene expression: (1) increased expression above control levels within one hour of cardioplegic arrest, with further amplification during early reperfusion; (2) increased expression limited to the reperfusion phase; and (3) reduced expression during reperfusion. Experiment Overall Design: 4 replicates each: Experiment Overall Design: Stage2, End-ischemia Experiment Overall Design: Stage1, Immediately post-cardioplegic arrest Experiment Overall Design: Stage3, After 5 min. Reperfusion

ORGANISM(S): Homo sapiens

SUBMITTER: Sara Arab

PROVIDER: E-GEOD-6381 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Early gene expression profiles during intraoperative myocardial ischemia-reperfusion in cardiac surgery.

Arab Sara S Konstantinov Igor E IE Boscarino Cathy C Cukerman Eva E Mori Alessandro A Li Jia J Liu Peter P PP Redington Andrew N AN Coles John G JG

The Journal of thoracic and cardiovascular surgery 20070701 1

<h4>Objective</h4>The effects of cold cardioplegic arrest and reperfusion on human ventricular gene expression are unknown. We tested the hypothesis that intraoperative ischemia-reperfusion under conditions of blood cardioplegic arrest would induce a unique myocardial genomic profile indicative of a cardioprotective response.<h4>Methods</h4>Right ventricular samples were serially acquired during surgical repair of ventricular septal defect.<h4>Results</h4>Expression profiling revealed 3 patterns ...[more]

PMID: 17599489

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Project description:Right ventricular free wall (RVFW) pacing results in left ventricular dyssynchrony with early septal shortening followed by late lateral contraction that reciprocally stretches the septum. Dyssynchrony is disadvantageous to cardiac mechano-energetics, yet little is known about its molecular consequences. We tested the hypothesis that dyssynchrony selectively alters regional gene expression in mice, employing a novel miniature implantable cardiac pacemaker. Mice were subjected to 1-week overdrive RVFW pacing (720 min-1, baseline HR 520-620 min-1) to induce dyssynchrony (pacemaker: 3V lithium battery, rate programmable, 0.8 grams, bipolar lead). Electrical capture was confirmed by pulsed-wave Doppler at implantation and terminal study, and dyssynchrony by echocardiography. Gene expression from left ventricular septal and lateral-wall myocardium were assessed by microarray (dual-dye method, Agilent) using oligonucleotide probes and dye swap. Identical analysis was applied to 4 synchronously contracting controls. Of 22,000 genes surveyed, only 18 genes displayed significant (p<0.01) differential expression between septal/lateral walls exceeding 1.5-fold relative to any disparities in synchronous controls. These changes were confirmed by qPCR with excellent correlations. Most (16) of the genes showed greater septal expression. Of particular interest were 7 genes coding proteins involved with stretch responses, matrix remodeling, stem cell differentiation to myocyte lineage, and Purkinje fiber differentiation. One-week cardiac dyssynchrony triggers regional differential expression differences in relatively few select genes. Such analysis using a murine implantable pacemaker should facilitate molecular studies of cardiac dyssynchrony and help elucidate novel mechanisms by which stress/stretch stimuli due to dyssynchrony impact the normal and failing heart. Experiment Overall Design: Left ventricle segments from the mouse heart - septal and lateral, were isolated from 4 dyssynchronous mice that were kept separate. RNA from the synchronous mice were pooled into a control septal and lateral sample. Functional genomic analysis was conducted on these 10 RNA samples with fluorophore reversal, such that each sample was assayed on two different microarrays. Corresponding septal and lateral samples from the same heart were paired on the same microarray to measure the relative differences in gene expression between the different regions of the heart then differences were compared across multiple mice to find overlapping genes that were affected by the pacememaker implantation.

Dataset Information

Transcription profiling of human right ventricular samples acquired during cardiac surgery

Publications

Early gene expression profiles during intraoperative myocardial ischemia-reperfusion in cardiac surgery.

Similar Datasets

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