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Genome-wide analysis of chromatin occupancy of GATA1 and its short isoform GATA1s (ChIP-Seq)

ABSTRACT: We report ChIP-Seq data for GATA1 and the leukemia-associated short isoform GATA1s in G1ME cells, a Gata1-null cell line with both erythroid and megakaryocytic differentiation potential. We introduced HA-tagged GATA1 or GATA1s into G1ME cells via retroviral transduction. The cells were crosslinked at 48h post-transduction, and an HA antibody was used for chromatin immunoprecipitation (ChIP). ChIP and input samples were sequenced on Illumina GAII or GAIIx high-throughput sequencers. The data reveal a deficiency of chromatin occupancy by GATA1s, especially at genes involved in erythrocyte differentiation and function. Examinaton of chromatin occupancy of GATA1 and GATA1s in G1ME cells cultured in TPO.

ORGANISM(S): Mus musculus

SUBMITTER: Timothy Chlon

PROVIDER: E-GEOD-64327 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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