Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide analysis of the role of FOG-1 in GATA-1 chromatin occupancy


ABSTRACT: We report ChIP-Seq data for GATA-1 and the FOG-binding mutant of GATA-1 (GATA-1^V205G) in G1ME cells, a Gata1-null cell line with both erythroid and megakaryocytic differentiation potential. We introduced HA-tagged GATA-1 or V205G into G1ME cells via retroviral transduction. The cells were crosslinked at 48h post-transduction, and an HA antibody was used for chromatin immunoprecipitation (ChIP). ChIP and input samples were sequenced on Illumina GAII high-throughput sequencer. The data reveal GATA-1-specific and V205G-specific bidning sites, indicating that FOG-1 both faacilitates and prohibits GATA-1 chromatin occupancy in a context-dependent manner. Examinaton of chromatin occupancy of GATA-1 anda FOG-binding mutant of GATA-1 in G1ME cells cultured in TPO.

ORGANISM(S): Mus musculus

SUBMITTER: Timothy Chlon 

PROVIDER: E-GEOD-35644 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cofactor-mediated restriction of GATA-1 chromatin occupancy coordinates lineage-specific gene expression.

Chlon Timothy M TM   Doré Louis C LC   Crispino John D JD  

Molecular cell 20120705 4


GATA-1 and its cofactor FOG-1 are required for the differentiation of erythrocytes and megakaryocytes. In contrast, mast cell development requires GATA-1 and the absence of FOG-1. Through genome-wide comparison of the chromatin occupancy of GATA-1 and a naturally occurring mutant that cannot bind FOG-1 (GATA-1(V205G)), we reveal that FOG-1 intricately regulates the chromatin occupancy of GATA-1. We identified GATA1-selective and GATA-1(V205G)-selective binding sites and show that GATA-1, in the  ...[more]

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