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Transcriptome analysis of lungs from six different mouse strains after infection with highly pathogenic H5N1 influenza A virus [set 2]

ABSTRACT: Susceptible (DBA/2J, 129/SvImJ, A/J) and Resistant (SM/J, C57BL/6J, Balb/cJ) mouse strain were inoculated with a highly pathogenic H5N1 influenza A virus (A/Hong Kong/213/2003) for 72 hours or not infected (control animals). Differences in expression were analyzed and used to identify candidate genes and pathways that contributed to the difference in H5N1 pathogenesis in these two groups of mice. Female 6-8 weeks old animals were inoculated with H5N1 virus or not and 72 hours later the lungs were obtained and immediately homogenized in Trizol. The extracted RNA was submitted for Illumina Gene expression profiling.

ORGANISM(S): Mus musculus

SUBMITTER: Adrianus Boon

PROVIDER: E-GEOD-64799 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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H5N1 influenza virus pathogenesis in genetically diverse mice is mediated at the level of viral load.

Boon Adrianus C M AC Finkelstein David D Zheng Ming M Liao Guochun G Allard John J Klumpp Klaus K Webster Robert R Peltz Gary G Webby Richard J RJ

mBio 20110906 5

<h4>Unlabelled</h4>The genotype of the host is one of several factors involved in the pathogenesis of an infectious disease and may be a key parameter in the epidemiology of highly pathogenic H5N1 influenza virus infection in humans. Gene polymorphisms may affect the viral replication rate or alter the host's immune response to the virus. In humans, it is unclear which aspect dictates the severity of H5N1 virus disease. To identify the mechanism underlying differential responses to H5N1 virus in ...[more]

PMID: 21896679

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Transcriptome analysis of lungs from six different mouse strains after infection with highly pathogenic H5N1 influenza A virus [set 1]

Project description:Susceptible (DBA/2J, 129/SvImJ, A/J) and Resistant (SM/J, C57BL/6J, Balb/cJ) mouse strain were inoculated with a highly pathogenic H5N1 influenza A virus (A/Hong Kong/213/2003) for 24 and 168 hours. Uninfected control animals were included. Differences in expression were analyzed and used to identify candidate genes and pathways that contributed to the difference in H5N1 pathogenesis in these two groups of mice. Female 6-8 weeks old animals were inoculated with H5N1 virus or not and 24 and 168 hours later the lungs were obtained and immediately homogenized in Trizol. The extracted RNA was submitted for Illumina Gene expression profiling.

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Lung expression data from highly pathogenic H5N1 virus infected and uninfected mice

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Transcriptome analysis of lungs from six different mouse strains after infection with highly pathogenic H5N1 influenza A virus [set 2]

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A single N66S mutation in the PB1-F2 protein of influenza A virus increases virulence by inhibiting the early interferon response in vivo

Project description:This study used virological, histological, and global gene expression from an experimental murine model of influenza infection to study the contribution of a specific mutation in the PB1-F2 protein (PB1-F2 N66S) of influenza A to viral pathogenesis. 6-8 week old, wild-type, female, C57Bl/6 mice were inoculated individually with 30 μl (10^4 PFU) of virus (recombinant influenza A/WSN/33 carrying the PB1 gene segment from A/Hong Kong/156/97 (H5N1) or a PB1 mutant recombinant virus resulting in an amino acid change at position 66 in the PB1-F2 protein [N66S]) in phosphate-buffered saline (PBS) containing penicillin-streptomycin and bovine serum albumin (PBS-BA-PS). A total of 10^4 PFU of virus was given in all inoculations. Control mice were given PBS-BA-PS. Lung samples were taken for microarray analysis at 12h, 1d, 3d, and 5d post-infection (n=3 animals per group at each time point for virus infected animals; n=2 animals per time point for mock-infected animals).

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Transcriptome analysis of lungs from six different mouse strains after infection with highly pathogenic H5N1 influenza A virus

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Project description:A global genomics approach was used to identify patterns of immune dysregulation during H5N1 influenza virus infection as the host response, in particular hyperchemokinemia, is thought to contribute to the extreme pathology associated with this disease. Keywords: time course Ferrets were inoculated intranasally with 10(6) EID50 of either A/Vietnam/1203/04 (H5N1) or A/Panama/2007/99 (H3N2). At 2, 4 and 6 days post-infection (DPI), ferrets were euthanized and lung tissue was excised for RNA purification and subsequent gene expression analysis.

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