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RNA-seq in squamous cell carcinoma stem cells in the presence or absence of TGF-beta signaling

ABSTRACT: We performed RNA-seq on purified squamous cell carcinoma stem cells (SCC-SCs) from primary mouse skin tumors transduced with TGF-beta reporter. SCC-SCs were purified based on cell surface marker expression integrin alpha6 and CD44, after lineage negative selection, and separated by fluorescent TGF-beta reporter expression.

ORGANISM(S): Mus musculus

SUBMITTER: Naoki Oshimori

PROVIDER: E-GEOD-64867 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TGF-β promotes heterogeneity and drug resistance in squamous cell carcinoma.

Oshimori Naoki N Oristian Daniel D Fuchs Elaine E

Cell 20150201 5

Subsets of long-lived, tumor-initiating stem cells often escape cancer therapies. However, sources and mechanisms that generate tumor heterogeneity and drug-resistant cell population are still unfolding. Here, we devise a functional reporter system to lineage trace and/or genetic ablate signaling in TGF-β-activated squamous cell carcinoma stem cells (SCC-SCs). Dissecting TGF-β's impact on malignant progression, we demonstrate that TGF-β concentrating near tumor-vasculature generates heterogeneit ...[more]

PMID: 25723170

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RNA-seq in squamous cell carcinoma stem cells in the presence or absence of TGF-beta signaling

Project description:We performed RNA-seq on purified squamous cell carcinoma stem cells (SCC-SCs) from primary mouse skin tumors transduced with TGF-beta reporter.

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ETS Family Transcriptional Regulators Drive Chromatin Dynamics and Malignancy in Squamous Cell Carcinomas (RNA-Seq I)

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