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Gene expression of distinct lung dendritic cell subsets


ABSTRACT: Pulmonary dendritic cells are heterogenous cells comprise four distinct subsets including two conventional dendritic cell subsets, CD103+ and CD11bhiCD14lo cells, and two monocyte-derived dendritic cell subsets. Their functions in terms of migration and T cell activation are distinct, but genes regulating their features are to be determined. We used microarrays to identify a select set of genes that are expressed in conventinal dendritic cells and in monocyte-derived dendriti cells. Four distinct lung DC subsets were purified by flow cytometry-based sorting after inhalation of lipopolusaccharide and ovalbumin. Each subset has three replicates.

ORGANISM(S): Mus musculus

SUBMITTER: NIEHS Microarray Core 

PROVIDER: E-GEOD-64896 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Complement receptor C5aR1/CD88 and dipeptidyl peptidase-4/CD26 define distinct hematopoietic lineages of dendritic cells.

Nakano Hideki H   Moran Timothy P TP   Nakano Keiko K   Gerrish Kevin E KE   Bortner Carl D CD   Cook Donald N DN  

Journal of immunology (Baltimore, Md. : 1950) 20150313 8


Differential display of the integrins CD103 and CD11b are widely used to distinguish two major dendritic cell (DC) subsets in nonlymphoid tissues. CD103(+) DCs arise from FLT3-dependent DC precursors (preDCs), whereas CD11b(hi) DCs can arise either from preDCs or FLT3-independent monocytes. Functional characterization of these two lineages of CD11b(hi) DCs has been hindered by the lack of a widely applicable method to distinguish between them. We performed gene expression analysis of fractionate  ...[more]

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