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Transcription profiling of mouse Earlybird (rab3a D77G) mutants and knockout of Rab3a on gene expression in the cortex and hippocampus

ABSTRACT: The cortex and hippocampus were dissected from each individual mouse. Targets from three biological replicates of earlybird mutants and their wildtype littermates and six replicates of Rab3a knockouts and wildtype littermates were generated and the expression profiles were determined using Affymetrix MOE430 A and B arrays. Comparisons between the sample groups allow the identification of tissue specifically expressed genes and genes that are affected by mutations of Rab3a. Experiment Overall Design: Cortex and hippocampus from 3 biological replicates of earlybird mutants and wildtype littermates and 6 biological replicates of Rab3a knockouts and wildtype littermates were analyzed

ORGANISM(S): Mus musculus

SUBMITTER: Maja Bucan

PROVIDER: E-GEOD-6527 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Biochemical, molecular and behavioral phenotypes of Rab3A mutations in the mouse.

Yang S S Farias M M Kapfhamer D D Tobias J J Grant G G Abel T T Bućan M M

Genes, brain, and behavior 20070201 1

Ras-associated binding (Rab) protein 3A is a neuronal guanosine triphosphate (GTP)-binding protein that binds synaptic vesicles and regulates synaptic transmission. A mouse mutant, earlybird (Ebd), with a point mutation in the GTP-binding domain of Rab3A (D77G), exhibits anomalies in circadian behavior and homeostatic response to sleep loss. Here, we show that the D77G substitution in the Ebd allele causes reduced GTP and GDP binding, whereas GTPase activity remains intact, leading to reduced pr ...[more]

PMID: 16734774

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