Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human Pluripotent stem cells: Normal diploid line vs lines with deletions of Chr7

ABSTRACT: Mapping deletions of Chr7 in human iPSC and ESCs derived from patients cells through reprogramming or chromosome engineering Two-condition experiment, normal diploid hPSCs vs del7q-hPSCs

ORGANISM(S): Homo sapiens

SUBMITTER: Eirini Papapetrou

PROVIDER: E-GEOD-65386 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Pluripotent stem cells: Normal diploid line vs lines with deletions of Chr7

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-02-16 | E-GEOD-65410 | biostudies-arrayexpress

Human induced Pluripotent stem cells: Normal diploid line vs lines with deletions of Chr7

Project description:Mapping deletions of Chr7 in human iPSC derived from patients cells through reprogramming Two-condition experiment, normal diploid iPSCs vs del7q-iPSCs

2015-02-16 | E-GEOD-65387 | biostudies-arrayexpress

Human induced pluripotent stem cells: diploid, chr7q deletion, spontaneous chr7 correction

Project description:Gene expression analysis, a) comparing isogenic karyotypically normal iPSCs to del7q-iPSCs, b) comparing del7q-iPSCs to spontaneously corrected iPSCs. The chr7q deletion results in reduced expression levels of a large number of genes in the chr7q deleted region Two-condition experiment, del7q-iPSCs vs. isogenic normal iPSCs and del7q-iPSCs vs spontaneously corrected-iPSCs. Biological replicates: 3 control replicates, 3 del7q-iPSC replicates and 3 spontaneously corrected-iPSC replicates

2015-02-16 | E-GEOD-65215 | biostudies-arrayexpress

Human Pluripotent stem cells: Normal diploid line vs lines with deletions of Chr7

Project description:Mapping deletions of Chr7 in human iPSC and ESCs derived from patients cells through reprogramming or chromosome engineering

2015-02-16 | GSE65386 | GEO

Human induced Pluripotent stem cells: Normal diploid line vs lines with deletions of Chr7

Project description:Mapping deletions of Chr7 in human iPSC derived from patients cells through reprogramming

2015-02-16 | GSE65387 | GEO

Heparin promotes cardiac differentiation of human pluripotent stem cells in chemically defined albumin-free medium enabling consistent manufacture of cardiomyocytes

Project description:Cardiomyocytes can be differentiated from human pluripotent stem cells (hPSCs) in defined conditions, but efficient and consistent cardiomyocyte differentiation often requires expensive reagents such as B27 supplement or recombinant albumin. Using a chemically defined albumin-free (E8 basal) medium, we identified heparin as a novel factor that significantly promotes cardiomyocyte differentiation efficiency, and developed an efficient method to differentiate hPSCs into cardiomyocytes. The treatment of heparin helped cardiomyocyte differentiation consistently reach at least 80% purity (up to 95%) from more than 10 different hPSC lines in chemically defined DMEM/F-12 based medium on either Matrigel or defined matrices like Vitronectin and Synthemax. One of heparinâs main functions was to act as a WNT modulator that helped promote robust and consistent cardiomyocyte production. Our study provides an efficient, reliable, and cost-effective method for cardiomyocyte derivation from hPSCs that can be used for potential large-scale drug screening, disease modeling, and future cellular therapies. 12 human pluripotent stem cells (hPSCs) at three different cardiac differentiation times (0 Days, 3 Days, 6 Days, 10 Days) under different culture conditions (+/- Heparin, +/- IWP2).

2016-07-28 | E-GEOD-81711 | biostudies-arrayexpress

Pluripotent stem cells: Normal diploid line vs lines with deletions of Chr7

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-02-16 | GSE65410 | GEO

RNA-seq of yeast with human chr7 YACs

Project description:RNA-seq was performed of S. cerevisiae harboring human chr7 YACs in order to gauge the frequency and form of transcription in naïve DNA (i.e. DNA that did not evolve in the host cell)

2022-11-15 | GSE217781 | GEO

RNA-seq of yeast with human chr7 YACs

Project description:RNA-seq of yeast with human chr7 YACs

| PRJNA900534 | ENA

Chromosome wide analysis of parental allele specific chromatin and DNA methylation along mouse distal chr7

Project description:Imprinted genes are monoallelically expressed according to parental inheritance. The maternally and paternally inherited alleles are distinguished epigenetically by DNA methylation and histone modifications. Chromosome-wide Chromatin immunoprecipitation (ChIP) and MIRA analysis of MatDup.dist7 and PatDup.dist7 MEFs provided a panoramic map of reciprocal allele-specific histone modifications and DNA methylation at imprinted genes along distal chromosome 7 and 15. ChIP-chip and MIRA-chip was done to map histone modifications and DNA methylation along distal chr7 in the maternal allele and paternal allele in Matdup.dist7 and Patdup.dist7 MEFs, respectively, using Nimblegen tiling arrays for distal chr7.

2011-03-31 | E-GEOD-26891 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data