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Expression profile in Overexpression of GST,GST-Med3

ABSTRACT: We have used microarrays to study the gene expression changes in Overexpression of GST,GST-Med3 of yeast cells to detect how the overexpressioin may affect global gene expression. RNA samples were taken from yeast cells overexpressed GST or GST-med3 and hybridized to affymetrix microarrays: GST and GST-Med3

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Claes Gustafsson

PROVIDER: E-GEOD-65666 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mediator tail subunits can form amyloid-like aggregates in vivo and affect stress response in yeast.

Zhu Xuefeng X Chen Lihua L Carlsten Jonas O P JO Liu Qian Q Yang Junsheng J Liu Beidong B Gustafsson Claes M CM

Nucleic acids research 20150702 15

The Med2, Med3 and Med15 proteins form a heterotrimeric subdomain in the budding yeast Mediator complex. This Med15 module is an important target for many gene specific transcription activators. A previous proteome wide screen in yeast identified Med3 as a protein with priogenic potential. In the present work, we have extended this observation and demonstrate that both Med3 and Med15 form amyloid-like protein aggregates under H2O2 stress conditions. Amyloid formation can also be stimulated by ov ...[more]

PMID: 26138482

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