Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic response in colon of rat with increased dietary protein content

ABSTRACT: Increase of fecal hazardous compounds and decrease of butyrate-producing bacteria has been implicated to be detrimental to colonic health during weight loss by high-protein diet in human. The question remains that whether the change of luminal microenvironment were paralleled with the alteration of colonic function in vivo.To reveal the change of gene expression involved in metabolic or immmunological process, we have employed the whole genome microarray profiling to identify differentially expressed genes in rat dietary normal protein (20% protein) or high protein (45% protein) diet. Colonic epithelium were sampled in adult male wistar rats (weighing 180-200g) fed each diet for six weeks.Each group contained six replicates.A total of 181 transcripts were significantly changed: 88 genes were up-regulated and 93 genes were down-regulated in high protein compared with normal protein rats. Transcriptome analysis identified the upregulation of chemotaxis, TNF-Î± signal process, antigen presentation, apoptosis, glutathione S-transferase activity and downregulation of innate immune, O-linked glycosylation of mucin, oxidative phosphorylation.Expression of ten genes, including calmodulin1,acetyl-CoA acyltransferase 2,isovaleryl-CoA dehydrogenase and occludin, were quantified in the same RNA samples by real-time PCR, validating the microarray analysis. Colonic gene expression was measured in male wistar rat (weighing 180-200g) after dietary normal protein or high protein diet for six weeks. Six replicates in each group were analyzed.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Weiyun Zhu

PROVIDER: E-GEOD-65862 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The Colonic Microbiome and Epithelial Transcriptome Are Altered in Rats Fed a High-Protein Diet Compared with a Normal-Protein Diet.

Mu Chunlong C Yang Yuxiang Y Luo Zhen Z Guan Leluo L Zhu Weiyun W

The Journal of nutrition 20160203 3

<h4>Background</h4>A high-protein diet (HPD) can produce hazardous compounds and reduce butyrate-producing bacteria in feces, which may be detrimental to gut health. However, information on whether HPD affects intestinal function is limited.<h4>Objective</h4>The aim of this study was to determine the impact of an HPD on the microbiota, microbial metabolites, and epithelial transcriptome in the colons of rats.<h4>Methods</h4>Adult male Wistar rats were fed either a normal-protein diet (20% protei ...[more]

PMID: 26843585

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Dataset Information

Transcriptomic response in colon of rat with increased dietary protein content

Publications

The Colonic Microbiome and Epithelial Transcriptome Are Altered in Rats Fed a High-Protein Diet Compared with a Normal-Protein Diet.

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