Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

A human fetal transcriptional atlas

ABSTRACT: Gene expression is highly dynamic during fetal development and determines tissue specification and function. In humans, the transcriptional profile of different organs during development has not been systematically studied. However, understanding how a particular tissue acquires its tissue identity will give insight into the development and maturation of tissues from a developmental biology perspective. Next-generation sequencing (DeepSAGE) dataset of 111 RNA samples representing 21 different human fetal organs and the maternal endometrium at three timepoints (gestational ages) during first and second trimester development (W9, W16-18, W22)

ORGANISM(S): Homo sapiens

SUBMITTER: Matthias Roost

PROVIDER: E-GEOD-66302 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

KeyGenes, a Tool to Probe Tissue Differentiation Using a Human Fetal Transcriptional Atlas.

Roost Matthias S MS van Iperen Liesbeth L Ariyurek Yavuz Y Buermans Henk P HP Arindrarto Wibowo W Devalla Harsha D HD Passier Robert R Mummery Christine L CL Carlotti Françoise F de Koning Eelco J P EJ van Zwet Erik W EW Goeman Jelle J JJ Chuva de Sousa Lopes Susana M SM

Stem cell reports 20150528 6

Differentiated derivatives of human pluripotent stem cells in culture are generally phenotypically immature compared to their adult counterparts. Their identity is often difficult to determine with certainty because little is known about their human fetal equivalents in vivo. Cellular identity and signaling pathways directing differentiation are usually determined by extrapolating information from either human adult tissue or model organisms, assuming conservation with humans. To resolve this, w ...[more]

PMID: 26028532

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Project description:Background: The aim of this study is to improve our understanding of the mechanisms underlying the function of corticosteriods in the limb muscles of ICU patients with acute quadriplegic myopathy (AQM) by using a unique porcine ICU model, i.e., 5-day longitudinal experiments where animals are sedated, mechanically ventilated and exposed to factor triggering AQM that is hydrocortisone. Results: An increased expression of genes involved in kinase activity, cell cycle regulation and transcriptional regulation. A decreased expression in genes regulating heat shock proteins, cytoskeletal & sarcomeric and oxidative stress response were also apparent. Therefore, it appears that CS has an additive deleterious role in acute quadriplegic myopathywere also apparent. Conclusions: CS induced molecular mechanisms involving kinase activity and heat shock protein genes were forwarded as probable mechanisms underlying the decreased force generating capacity. Eight female domestic piglets (Sus scrofa, average body weight 26.4 kg) were used in this study. Piglets were immobilized by anesthesia and mechanically ventilated via a tracheotomy for a period of five days. During this study period, the animals were sedated using isoflurane inhalation (Abbott Laboratories, Chicago, Il, USA, 0.8 - 1.3% end-tidal concentration) supplemented by intravenous bolus doses of morphine and ketamine as needed. Core body temperature (blood) was maintained in the range of 38.5 - 40M-BM-0C by a servo controlled heating pad. The animals received intravenous crystalloid fluid (Ringeracetat) to maintain stable blood pressure and urinary output and a glucose infusion (Rehydrex, Fresenius Kabi, Stockholm, Sweden, 25 mg glucose /mL) in the range of 0.5 - 1.5 mg/kg/minute to decrease the effects of catabolism. Each animal received prophylactic streptomycin 750 mg/d and bensylpenicillin 600 mg/d (Streptocillin Vet, Boeringer-Ingelheim, Hellerup, Denmark). Arterial blood gas analysis as well as electrolytes and blood glucose levels were monitored regularly and kept in the normal range throughout the study period. A neuromuscular blocking agent (NMBA) was administered as a continuous infusion of pancuronium bromide 0.1mg/kg/h (Pavolun; Organon, Boxtel, The Netherlands) for 5days while a corticosteroid (CS) was given as bolus doses of hydrocortisone. Keywords: Treatment, immobilization, muscle function.

Dataset Information

A human fetal transcriptional atlas

Publications

KeyGenes, a Tool to Probe Tissue Differentiation Using a Human Fetal Transcriptional Atlas.

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