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Interferon-Î³ response against C. burnetii of peripheral blood mononuclear cells in the development of chronic Q fever

ABSTRACT: Background: Q fever is caused by the Coxiella burnetii, an intracellular bacterium that infects mononuclear cells. In some individuals, it causes a persistent cardiovascular infection (chronic Q fever). The aim of present study was to investigate the C. burnetii-induced IFN-Î³ response in chronic Q fever patients. Methods: IFN-Î³ was measured in supernatants of C. burnetii-stimulated peripheral blood mononuclear cells (PBMCs) of patients. Gene-expression profiles of the IFN-Î³ pathway in PBMCs after incubation with C. burnetii were compared between chronic Q fever patients and control individuals. Results: IFN-Î³ production by PBMCs of chronic Q fever patients incubated with C. burnetii in vitro, was significantly higher compared to controls. In transcriptome analysis, genes downstream of IFN-Î³ were strongly upregulated in patients. Conclusion: Present study showed that IFN-Î³ production and the response to IFN-Î³ seems to be intact in chronic Q fever patients. PBMC were purified from Q fever patients (n=6) or healthy volunteers (n=4), and then stimulated by Coxiella burnetii, LPS or left untreated (NS)

ORGANISM(S): Homo sapiens

SUBMITTER: Julien Textoris

PROVIDER: E-GEOD-66476 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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