Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of differences in the transcriptome of WAT from Wildtype and 14-3-3zeta knockout mice

ABSTRACT: Due to inherent differences in adipcoyte size between wildtype and knockout animals, we sought to examine if the decrease in adipocyte size was due to differences in the transcriptome and more specifcially, adipogenic genes. Examination of the transcriptome in wildtype (WT) and knockout (KO) gonadal white adipose tissue from adult mice

ORGANISM(S): Mus musculus

SUBMITTER: Gareth Lim

PROVIDER: E-GEOD-66716 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Analysis of differences in the transcriptome of WAT from Wildtype and 14-3-3zeta knockout mice

Project description:Due to inherent differences in adipcoyte size between wildtype and knockout animals, we sought to examine if the decrease in adipocyte size was due to differences in the transcriptome and more specifcially, adipogenic genes.

2015-07-23 | GSE66716 | GEO

SYSTEMATIC TRANSCRIPTIONAL PROFILING OF THE LIVER, HEART, SKELETAL MUSCLE, AND ADIPOSE TISSUE IN INSULIN RESISTANT, DIET-INDUCED OBESE C57BL/6J MOUSE

Project description:Following 20wk of either chow or high fat feeding, tissues were collected from C57BL/6J mice. Gene expression in four tissues that have been associated with obesity-related metabolic complications: white adipose tissue (WAT), skeletal muscle, the liver, and the heart were examined. Data suggest that some tissues may be attempting to reduce the metabolic perturbations that occur in the early stages of obesity. Also, many identified genes were associated with a variety of disorders, thereby serving as potential links between obesity and related health risks. After 3 wk of weaning, male C57BL/6J littermates were randomly separated into control (n=6) and treatment (n=6) groups for each tissue. After an acclimatization period of 1wk, animals were given free access to a chow diet (23% protein, 21% fat, and 55% carbohydrate) or a high fat (HF) diet (15% protein, 59% fat, and 26% carbohydrate) for 20wk. Following the feeding period, animals were fasted for 6h, anesthetized with pentobarbital, and weighed. Blood glucose was assessed in anesthetized mice. Non-esterified fatty acids and immunoreactive insulin was assayed with a double antibody method. Samples of WAT, the liver, gastrocnemius muscle and heart were obtained, frozen with liquid nitrogen, and stored at -80oC until subsequent use. Tissue samples were homogenized at short intervals of time and total RNA was isolated using cold TRIzol (Invitrogen, Ontario, Canada) to prevent RNA degradation. Cdna was prepared to perform the microarrays. For every treatment sample and its corresponding control, two microarrays were performed in a dye-swap normalization experiment. Mouse M7K microarray chip from the Southern Alberta Microarray Facility (University of Calgary, Calgary, AB) were used (GEO Accession: Platform GPL3965).

2011-03-01 | E-GEOD-26029 | biostudies-arrayexpress

Comparison of WAT EPCs vs WAT ASCs

Project description:Human adipose tissue contains two populations of progenitors (EPCs and ASCs) with cooperative roles in breast cancer. EPCs (CD45-CD34+CD31+CD13-CCRL2+) can generate endothelial cells. ASCs (CD45-CD34+CD31-CD13+CD140b+) are mesenchymal progenitors which generated pericytes. CD13+ cells and CD13- cells from 7 Lipotransfer aspirate

| S-ECPF-GEOD-44302 | biostudies-other

NUDT2 Knockout full proteome mouse

Project description:Proteome comparison between bone-marrow cells isolated from wildtype and NUDT2 knockout mice.

2021-11-03 | PXD023219 | Pride

ISR target genes in WAT and BAT of mAP::Fv2E-PERK

Project description:To identify the target genes of integrated stress reponse (ISR) in WAT and BAT, we have employed whole genome microarray expression in WAT and BAT specific Fv2E-PERK transgenic mice. The mAP::Fv2E-PERK transgenic mice were injected by AP20187 or mock.

2015-08-05 | E-GEOD-49645 | biostudies-arrayexpress

Expression data from Adam17 knock out mice and wild type

Project description:Adam17, a shedding protease, is strongly upregtulated during inflammation and cancer. Here we investigate the genome wide effects of Adam17 knock out on the transcriptome. Mouse tissue samples from n=3 wildtype colon, n=3 Adam17 knockout colon, n=3 wildtype skin and n=3 Adam17 knockout skin were used in the study.

2010-07-08 | E-GEOD-21691 | biostudies-arrayexpress

Detection of putative PKA target phosphorylation sites in Atg5flox:CamKII-Cre wildtype and knockout mice brain tissue

Project description:The experiment was performed to identify PKA phosphorylation substrates in wildtype and autophagy-deficient brains. Therefore, hippocampus and cortex of wildtype and conditional knockout mice were isolated and sliced. The brain slices were incubated with DMSO or Forskolin, a cAMP elevating agent, to induce PKA activity. Samples were measured by LC-MS/MS and were used to quantify proteomic and phosphoproteomic changes.

2022-11-15 | PXD031106 | Pride

14-3-3ζ controls adipocyte progenitor cell cycle and differentiation via Gli3-dependent p27Kip expression

Project description:14-3-3 proteins facilitate cytoplasmic-nuclear shuttling of transcription factors.Adipocyte differentiation requires the function of critical transcription factors to drive the development of a mature adipocyte. The aim of the study was to investigate if 14-3-3ζ is required for the adipogenic transcriptional program. Examination of the transcriptome in siCon- and si14-3-3ζ-transfected 3T3-L1 cells undergoing differentiation at t=0, 24, and 48 hours.

2015-07-23 | E-GEOD-60745 | biostudies-arrayexpress

Global proteome of Atg5flox:CAG-CreTmx WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient primary neurons. Therefore, cortico-hippocampal neurons were isolated from Atg5flox:CAG-CreTmx mice and treated with Tamoxifen (Tmx) to induce the knockout (KO) or EtOH for wildtype (WT) in-vitro. WT and KO Neurons were harvested at day in-vitro (DIV) 15-16, and global proteome analysis was measured by LC-MS/MS.

2022-11-15 | PXD031487 | Pride

Global proteome of Atg16L1flox:CAG-CreTmx WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient primary neurons. Therefore, cortico-hippocampal neurons were isolated from Atg16L1flox:CAG-CreTmx mice and treated with Tamoxifen (Tmx) to induce the knockout (KO) or EtOH for wildtype (WT) in-vitro. WT and KO Neurons were harvested at day in-vitro (DIV) 15-16, and global proteome analysis was measured by LC-MS/MS.

2022-11-15 | PXD031555 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data