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Identifying PD0325901-regulated genes in the J1 mESCs

ABSTRACT: It has been demonstrated that PD0325901 promotes self-renewal of mouse embryonic stem cells, however, the target genes of PD0325901 are not fully understood. To better understand the downstream target genes of PD0325901, we performed Microarray analyses to identify their downstream targets. The data show the genes regulated by PD0325901. J1 mESCs maintained in medium containing 1000 U/mL LIF and supplemented without or with PD0325901 for 24 hours, then total RNA was extracted for analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Zhiying Ai

PROVIDER: E-GEOD-67534 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901.

Ai Zhiying Z Shao Jingjing J Shi Xinglong X Yu Mengying M Wu Yongyan Y Du Juan J Zhang Yong Y Guo Zekun Z

Stem cells international 20151207

Embryonic stem cells (ESCs) have the ability to grow indefinitely and retain their pluripotency in culture, and this self-renewal capacity is governed by several crucial molecular pathways controlled by specific regulatory genes and epigenetic modifications. It is reported that multiple epigenetic regulators, such as miRNA and pluripotency factors, can be tightly integrated into molecular pathways and cooperate to maintain self-renewal of ESCs. However, mouse ESCs in serum-containing medium seem ...[more]

PMID: 26770202

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