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A survey of human brain transcriptome diversity at the single cell level

ABSTRACT: We used single cell RNA sequencing on 466 cells to capture the cellular complexity of the adult and fetal human brain at a whole transcriptome level. Healthy adult temporal lobe tissue was obtained from epileptic patients during temporal lobectomy for medically refractory seizures. We were able to classify individual cells into all of the major neuronal, glial, and vascular cell types in the brain. Examination of cell types in healthy human brain samples.

ORGANISM(S): Homo sapiens

SUBMITTER: Martin Enge

PROVIDER: E-GEOD-67835 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A survey of human brain transcriptome diversity at the single cell level.

Darmanis Spyros S Sloan Steven A SA Zhang Ye Y Enge Martin M Caneda Christine C Shuer Lawrence M LM Hayden Gephart Melanie G MG Barres Ben A BA Quake Stephen R SR

Proceedings of the National Academy of Sciences of the United States of America 20150518 23

The human brain is a tissue of vast complexity in terms of the cell types it comprises. Conventional approaches to classifying cell types in the human brain at single cell resolution have been limited to exploring relatively few markers and therefore have provided a limited molecular characterization of any given cell type. We used single cell RNA sequencing on 466 cells to capture the cellular complexity of the adult and fetal human brain at a whole transcriptome level. Healthy adult temporal l ...[more]

PMID: 26060301

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