Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq as a global measure of the similarity between human pluripotent stem cell and fetal liver derived B cell hierarchies

ABSTRACT: Evidence suggests childhood acute lymphoblastic leukemia (cALL) arises in early human development. Existing models of pre-leukemic initiation using the ETV6-RUNX1 fusion do not recapitulate human disease, highlighting the need for a developmentally relevant human model system. A human pluripotent stem cell (hPSC) model genome was engineered to express ETV6-RUNX1 from the endogenous ETV6 promoter. RNA-seq data from sorted hematopoietic progenitors identified according to surface markers.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Chela James 

PROVIDER: E-MTAB-6382 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1.

Böiers Charlotta C   Richardson Simon E SE   Laycock Emma E   Zriwil Alya A   Turati Virginia A VA   Brown John J   Wray Jason P JP   Wang Dapeng D   James Chela C   Herrero Javier J   Sitnicka Ewa E   Karlsson Stefan S   Smith Andrew J H AJH   Jacobsen Sten Erik W SEW   Enver Tariq T  

Developmental cell 20171228 3

ETV6-RUNX1 is associated with childhood acute B-lymphoblastic leukemia (cALL) functioning as a first-hit mutation that initiates a clinically silent pre-leukemia in utero. Because lineage commitment hierarchies differ between embryo and adult, and the impact of oncogenes is cell-context dependent, we hypothesized that the childhood affiliation of ETV6-RUNX1 cALL reflects its origins in a progenitor unique to embryonic life. We characterize the first emerging B cells in first-trimester human embr  ...[more]

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