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SiRNA profiling of human cells knocked down for optineurin

ABSTRACT: This study aimed to identify genes that are linked with optineurin expression using a combined siRNA-microarray approach Experiment Overall Design: Samples a01, a02 and a03 were treated with siRNA specific for optineurin mRNA Experiment Overall Design: Samples b01, b02 and b03 were treated with water (mock experiment)

ORGANISM(S): Homo sapiens

SUBMITTER: Nicole Weisschuh

PROVIDER: E-GEOD-6819 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of genes that are linked with optineurin expression using a combined RNAi--microarray approach.

Weisschuh Nicole N Alavi Marcel V MV Bonin Michael M Wissinger Bernd B

Experimental eye research 20070627 4

Mutations in the optineurin gene are associated with open-angle glaucoma. Its gene product is a 74 kDa protein implicated in several cellular pathways. Although a range of interacting partners of optineurin have been identified, its physiological and pathophysiological role remains unclear. To understand comprehensive molecular mechanisms by which optineurin mediates, we identified genome-wide molecular changes upon silencing optineurin in HeLa cells by using microarray technology. A series of d ...[more]

PMID: 17663987

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