Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Effect of high-fat diet (HFD) on gene expression in brown adipose tissue (BAT)


ABSTRACT: C57BL/6 male mice were fed a standardized NCD or HFD after weaning (4 weeks of age) over a course of 12 weeks. Normal chow diet (NCD) fed animals served as Controls Biotin-labeled cDNA was synthesized using GeneChip Whole Transcript Sense Labeling Assay (Affymetrix) according to vendor’s instructions. After fragmentation, cDNAs were hybridized for 17h at 45°C on Affymetrix Mouse Gene 1.0 ST Arrays. The Arrays were washed and stained in the GeneChip Fluidics Station 450 and scanned on a GeneChip Scanner 3000 7G (Affymetrix). Data intensities were log transformed and normalized with a quantile normalization method using Affymetrix Power Tools. Differentially expressed genes were identified according to statistical evidence indicated by Student's t-test and fold change statistics

ORGANISM(S): Mus musculus

SUBMITTER: Jan-Wilhelm Kornfeld 

PROVIDER: E-GEOD-69608 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2015-06-06 | GSE69608 | GEO
2012-12-16 | E-GEOD-35497 | biostudies-arrayexpress
2014-08-11 | E-GEOD-41104 | biostudies-arrayexpress
2012-12-16 | GSE35497 | GEO
2017-10-21 | GSE105448 | GEO
2012-11-10 | E-GEOD-42188 | biostudies-arrayexpress
2022-08-19 | GSE207856 | GEO
2023-09-05 | GSE241781 | GEO
2023-07-03 | GSE234889 | GEO
2018-08-30 | PXD010209 | Pride