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A fully validated blood stem/progenitor cell regulatory network reveals mechanisms of cell state stabilisation

ABSTRACT: The aim of this study was to determine the genomic binding sites of important haematopoietic transcription factors in haematopoietic cell lines. 416Bs cells were fixed with either 1 or 0.4 % formaldehyde for 10 mins. Chromatin was isolated, sonicated for 7 mins (30 sec on, 30 sec off), and specific antibodies were used to pull down the transcription factors of interest after a pre-clearing step. Chromatin was washed, de-crosslinked, amplified, size selected by gel purification and sequenced.

ORGANISM(S): Mus musculus

SUBMITTER: Rebecca Hannah

PROVIDER: E-GEOD-69776 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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