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Cytokine-mediated differentiation reflects genome-wide loss of a uSTAT transcriptional program

ABSTRACT: uSTAT transcriptional program HPC7 cells were fixed with either 1% formaldehyde for 10 mins. Chromatin was isolated, sonicated for 7 mins (30 sec on, 30 sec off), and specific antibodies were used to pull down the transcription factors of insterest after a pre-clearing step. Chromatin was washed, de-crosslinked, amplified, size selected by gel purification and sequenced.

ORGANISM(S): Mus musculus

SUBMITTER: Rebecca Hannah

PROVIDER: E-GEOD-70697 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cytokine-induced megakaryocytic differentiation is regulated by genome-wide loss of a uSTAT transcriptional program.

Park Hyun Jung HJ Li Juan J Hannah Rebecca R Biddie Simon S Leal-Cervantes Ana I AI Kirschner Kristina K Flores Santa Cruz David D Sexl Veronika V Göttgens Berthold B Green Anthony R AR

The EMBO journal 20151223 6

Metazoan development is regulated by transcriptional networks, which must respond to extracellular cues including cytokines. The JAK/STAT pathway is a highly conserved regulatory module, activated by many cytokines, in which tyrosine-phosphorylated STATs (pSTATs) function as transcription factors. However, the mechanisms by which STAT activation modulates lineage-affiliated transcriptional programs are unclear. We demonstrate that in the absence of thrombopoietin (TPO), tyrosine-unphosphorylated ...[more]

PMID: 26702099

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