Comparison of globin RNA processing methods for genome-wide transcriptome analysis from whole-blood

ABSTRACT: Whole blood rather than purified peripheral blood mononuclear cells is likely to become the prime tissue using expression microarrays for disease predication or prognosis however excess of globin mRNA may reduce probe detection sensitivity. In our study, we assessed whether whole-blood or globin-reduced RNA gives the most robust and sensitive results to detect small gene expression changes in response to hormone replacement therapy exposure. Each sample (N = 12) were hybridized according to 3 different protocols: no globin reduction (controls), globin reduction using peptid nucleic acids (PNA) and using magnetic beads in the GlobinClear kit from Ambion. Finally, 7 and 4 technical replicates were conducted in no globin reduction and PNA groups, respectively. Both globin reduction approaches

ORGANISM(S): Homo sapiens

SUBMITTER: Vanessa Dumeaux 

PROVIDER: E-GEOD-7008 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress