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Dietary selenium levels affect selenoprotein expression and support the interferon-γ and IL-6 immune response pathways in mice [RNA-Seq]

ABSTRACT: Mice were fed Se-deficient or Se-adequate diets for 6 weeks. Liver and lung tissue were harvested and processed for RNA-Seq, ribosome profiling, and microarray analysis. From these studies, we identified changes in mRNA levels and translation of selenoprotein genes and genes regulated by interferon-gamma. Cytokine profiles of serum indicated that interferon-gamma and IL-6 levels were increased in the Se-adequate mice relative to Se-deficient mice. RNA-Seq analysis of liver tissue from mice fed Se-deficient or Se-adequate diets

ORGANISM(S): Mus musculus

SUBMITTER: Michael Howard

PROVIDER: E-GEOD-70158 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dietary selenium levels affect selenoprotein expression and support the interferon-? and IL-6 immune response pathways in mice [microarray]

Project description:Mice were fed Se-deficient or Se-adequate diets for 6 weeks. Liver and lung tissue were harvested and processed for RNA-Seq, ribosome profiling, and microarray analysis. From these studies, we identified changes in mRNA levels and translation of selenoprotein genes and genes regulated by interferon-gamma. Cytokine profiles of serum indicated that interferon-gamma and IL-6 levels were increased in the Se-adequate mice relative to Se-deficient mice. Gene expression analysis of liver and lung tissue from mice fed Se-deficient or Se-adequate diets

2015-08-06 | E-GEOD-71716 | biostudies-arrayexpress

Dietary selenium levels affect selenoprotein expression and support the interferon-γ and IL-6 immune response pathways in mice [Ribosome Profiling]

Project description:Mice were fed Se-deficient or Se-adequate diets for 6 weeks. Liver and lung tissue were harvested and processed for RNA-Seq, ribosome profiling, and microarray analysis. From these studies, we identified changes in mRNA levels and translation of selenoprotein genes and genes regulated by interferon-gamma. Cytokine profiles of serum indicated that interferon-gamma and IL-6 levels were increased in the Se-adequate mice relative to Se-deficient mice. Ribosome profiling of liver tissue from mice fed Se-deficient or Se-adequate diets

2015-08-06 | E-GEOD-70159 | biostudies-arrayexpress

Dietary selenium levels affect selenoprotein expression and support the interferon-γ and IL-6 immune response pathways in mice [microarray]

2015-08-06 | GSE71716 | GEO

Dietary selenium levels affect selenoprotein expression and support the interferon-γ and IL-6 immune response pathways in mice [Ribosome Profiling]

2015-08-06 | GSE70159 | GEO

Dietary selenium levels affect selenoprotein expression and support the interferon-γ and IL-6 immune response pathways in mice [RNA-Seq]

2015-08-06 | GSE70158 | GEO

Selenium toxicity but not deficient or super-nutritional selenium status vastly alters the transcriptome in rodents

Project description:Protein and mRNA levels for several selenoproteins, such as glutathione peroxidase-1 (Gpx1), are down-regulated dramatically by selenium (Se) deficiency. Selenoprotein levels in rats increase sigmoidally with increasing dietary Se and reach defined plateaus at the Se requirement, making them sensitive biomarkers for Se deficiency, but not high for Se status. Biomarkers for high Se status are needed as super-nutritional Se intakes are associated with beneficial and adverse health outcomes, but conventional biomarkers are not especially useful above the Se requirement. To characterize Se regulation of the transcriptome, we conducted 3 microarray experiments in weanling mice and rats fed Se-deficient diets supplemented with levels of Se up to 5 µg Se/g diet. Rats or mice were fed Se-deficient diets supplemented with sodium selenite up to 5 ug Se/g diet for 28 or 35 days. Affymetrix Rat 230 2.0 and Mouse 430 2.0 Genome Arrays were used to analyze gene expression in liver in all studies plus kidney in the mouse study.

2011-01-19 | E-GEOD-23895 | biostudies-arrayexpress

Mouse Iron Distribution - Rich and Deficient iron diets (tracer)

Project description:Mouse Iron Distribution Dynamics Dynamic model of iron distribution in mice. This model attempts to fit the radioiron tracer data from Lopes et al. 2010 for mice fed iron deficient and rich diets by adjusting the rate of iron intake (vDiet) and the hepcidin synthesis rate (vhepcidin) independently for each experiment. All other parameters are those that provide the best fit for the adequate diet. This model includes the radioiron tracer species. Differences in parameter values between deficient, rich, and adequate diets: Diet vDiet vhepcidin Adequate 0.00377422 1.7393e-08 Deficient 0 8.54927e-09 Rich 0.00415624 2.30942e-08

2024-09-02 | BIOMD0000000734 | BioModels

Selenium toxicity but not deficient or super-nutritional selenium status vastly alters the transcriptome in rodents

2011-01-19 | GSE23895 | GEO

The effects of dietary selenium on selenocysteine incorporation and selenoprotein expression

Project description:The goal of this study was to determine the effects of dietary selenium levels on translational control of selenoprotein synthesis in mouse liver. Wild type mice and mice expressing a mutant Sec-tRNA gene (TrspA37G) were fed diets supplemented with 0, 0.1, or 2 ppm selenium for 6 weeks. Livers were harvested and ribosome and mRNA profiles were generated by deep-sequencing using the Illumina HiSeq 2000.

2013-09-17 | E-GEOD-46038 | biostudies-arrayexpress

Four selenoproteins, protein biosynthesis, and Wnt signalling are particularly sensitive to limited selenium intake in mouse colon

Project description:Selenium is an essential micronutrient. Its recommended daily allowance is not attained by a significant proportion of the population in many countries and its intake has been suggested to affect colorectal carcinogenesis. Therefore, microarrays were used to determine how both selenoprotein and global gene expression patterns in the mouse colon were affected by marginal selenium deficiency comparable to variations in human dietary intakes. Two groups of 12 mice each were fed a selenium-deficient (0.086mg Se/kg) or a selenium-adequate (0.15mg Se/kg) diet. After 6wk, plasma selenium level, liver, and colon glutathione peroxidase (GPx) activity in the deficient group was 12, 34, and 50%, respectively, of that of the adequate group. Differential gene expression was analysed with mouse 44K whole genome microarrays. Pathway analysis by GenMAPP identified the protein biosynthesis pathway as most significantly affected, followed by inflammation, Delta-Notch and Wnt pathways. Selected gene expression changes were confirmed by quantitative real-time PCR. GPx1 and the selenoproteins W, H, and M, responded significantly to selenium intake making them candidates as biomarkers for selenium status. Thus, feeding a marginal selenium-deficient diet resulted in distinct changes in global gene expression in the mouse colon. Modulation of cancer-related pathways may contribute to the higher susceptibility to colon carcinogenesis in low selenium status.

2018-07-13 | GSE117025 | GEO

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