Unknown,Transcriptomics,Genomics,Proteomics

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Whole Genome Bisulfite sequencing of Arabidopsis thaliana High and Low phosphate methylomes


ABSTRACT: Pi availability is a significant limiting factor for plant growth in both natural and agricultural systems. To cope with such limiting conditions, plants have adapted developmental and biochemical strategies to enhance Pi acquisition and to avoid starvation. A myriad of genes that are involved in the regulation and display of these strategies have been identified. However, the possible epigenetic components regulating the phosphate starvation responses have not been thoroughly investigated. DNA methylation is a major epigenetic mark involved in diverse biological processes and it may play a critical role in Pi starvation stress adaptation, also changes in DNA methylation can lead to a unique gene expression pattern in response to specific developmental and environmental conditions. Here in we demonstrate that non-CpG DNA methylation is required for proper expression of a number of Pi-limitation responsive genes in Arabidopsis thaliana and results in altered morphologic and physiologic phosphate starvation responses.Our data suggest that DNA methylation is involved in the modulation of Pi starvation responses via the transcriptional regulation of a set of phosphate-starvation responsive genes. Analysis of 8 different treatments, 2 different Organs (Root and Shoot), 2 different Phosphate treatments (High Pi, Low Pi), 2 different Times (Short Term, Long Term), 2 biological replicates for treatment

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Luis Rafael Estrella 

PROVIDER: E-GEOD-72770 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Methylome analysis reveals an important role for epigenetic changes in the regulation of the Arabidopsis response to phosphate starvation.

Yong-Villalobos Lenin L   González-Morales Sandra Isabel SI   Wrobel Kazimierz K   Gutiérrez-Alanis Dolores D   Cervantes-Peréz Sergio Alan SA   Hayano-Kanashiro Corina C   Oropeza-Aburto Araceli A   Cruz-Ramírez Alfredo A   Martínez Octavio O   Herrera-Estrella Luis L  

Proceedings of the National Academy of Sciences of the United States of America 20151214 52


Phosphate (Pi) availability is a significant limiting factor for plant growth and productivity in both natural and agricultural systems. To cope with such limiting conditions, plants have evolved a myriad of developmental and biochemical strategies to enhance the efficiency of Pi acquisition and assimilation to avoid nutrient starvation. In the past decade, these responses have been studied in detail at the level of gene expression; however, the possible epigenetic components modulating plant Pi  ...[more]

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