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Transcription profiling of mouse TfRc deficient and wild type renalcells in chimeric embryonic kidneys derived from embryos created by blastocyst injection

ABSTRACT: We investigated the ability of transferrin receptor1 (TfRc) knockout cells to populate different domains of the developing kidney by using a chimeric approach. The TfRc cells developed into all segments of the developing nephron, but there was a relative exclusion from the ureteric bud and a positive bias towards the stromal compartment. Here we conducted a microarray analysis of differential gene expression between TfRc deficient and wild type (wt) cells in chimeric embryonic kidneys derived from embryos created by blastocyst injection of wt blastocysts with TfRc-/- green fluorescent protein-expressing (GFP+) embryonic stem cells. Experiment Overall Design: Following blastocyst injection of 3-5 TfRc-/- GFP+ embryonic stem cells in wt blstocysts, we harvested the chimeric kidney at day E15.5 and separated the TfRc-/- GFP+ cell population from wt GFP- cell population by using fluorescence activated cell sorting. Biotinlyated cRNA was prepared from the isolated cells and hybridized to Mouse Genome 430 2.0 Arrays (Affymetrix) according to standard protocols.

ORGANISM(S): Mus musculus

SUBMITTER: Jonathan Barasch

PROVIDER: E-GEOD-7396 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TfRc Dependent Gene Expression in Embryonic Renal Cells

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Single-cell RNAseq of control chimeric mouse embryos at embryonic days 7.5 and 8.5 of mouse development

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Transcription profiling of mouse wild type embryonic stem cells after blastocytst injection revealglobal corrections in MDX mice.

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2008-10-25 | E-GEOD-12580 | biostudies-arrayexpress

Blastocyst Injection of Wild Type Embryonic Stem Cells Induces Global Corrections in Mdx Mice.

2008-08-30 | GSE12580 | GEO

Gene expression profiles of the E15.5 developing podocyte (GUDMAP Series_id: 33)

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