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Single-cell RNAseq of control chimeric mouse embryos at embryonic days 7.5 and 8.5 of mouse development

ABSTRACT: Chimeric mouse embryos were generated as a control experiment to understand the contribution of host vs. injected cells to the developing embryo. Embryos were generated by blastocyst injection of tdTomato-labelled mouse embryonic stem cells into wild type embryos. After blastocyst harvest, cells were flow-sorted before 10X Genomics library preparation and single-cell RNA-sequencing.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Carolina Guibentif

PROVIDER: E-MTAB-7324 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A single-cell molecular map of mouse gastrulation and early organogenesis.

Pijuan-Sala Blanca B Griffiths Jonathan A JA Guibentif Carolina C Hiscock Tom W TW Jawaid Wajid W Calero-Nieto Fernando J FJ Mulas Carla C Ibarra-Soria Ximena X Tyser Richard C V RCV Ho Debbie Lee Lian DLL Reik Wolf W Srinivas Shankar S Simons Benjamin D BD Nichols Jennifer J Marioni John C JC Göttgens Berthold B

Nature 20190220 7745

Across the animal kingdom, gastrulation represents a key developmental event during which embryonic pluripotent cells diversify into lineage-specific precursors that will generate the adult organism. Here we report the transcriptional profiles of 116,312 single cells from mouse embryos collected at nine sequential time points ranging from 6.5 to 8.5 days post-fertilization. We construct a molecular map of cellular differentiation from pluripotency towards all major embryonic lineages, and explor ...[more]

PMID: 30787436

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Single-cell RNAseq of additional control chimeric mouse embryos at embryonic day 8.5 of mouse development

Project description:This dataset contains additional data performed as in the experiment stored at accession E-MTAB-7324. Chimeric mouse embryos were generated as a control experiment to understand the contribution of host vs. injected cells to the developing embryo. Embryos were generated by blastocyst injection of tdTomato-labelled mouse embryonic stem cells into wild type embryos. After blastocyst harvest, cells were flow-sorted before 10X Genomics library preparation and single-cell RNA-sequencing.

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Single-cell RNAseq of Tal1 knockout chimeric mouse embryos at embryonic days 7.5 and 8.5 of mouse development.

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Single-cell RNAseq of Brachyury knockout chimeric mouse embryos at embryonic days 8.5 and 7.5 of mouse development.

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NPKLH data

Project description:Comparison of the germinal center B cell receptor repertoire in Cxcl13-Cre/TdTom EYFP (control) and Cxcl13-Cre/TdTom EYFP Cxcl12fl/fl mice on day following subcutaneous immunization with NP-KLH.

2020-05-12 | E-MTAB-8904 | biostudies-arrayexpress

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2020-05-12 | E-MTAB-8445 | biostudies-arrayexpress

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