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Single-cell RNAseq of Tal1 knockout chimeric mouse embryos at embryonic day 8.5 of mouse development

ABSTRACT: Chimeric embryos were generated to investigate the effect of Tal1 knockout in mouse embryos by single-cell RNA-sequencing. Tal1 is an essential transcription factor for the formation of the embryonic blood. Embryo chimerism permits the analysis of the effects of Tal1 knockout without the confounding effects of the absence of embryonic blood, which results in global developmental failures. Embryos were generated by blastocyst injection of tdTomato-labelled, Tal1-/- mouse embryonic stem cells into wild type embryos. After blastocyst harvest, cells were flow-sorted before 10X Genomics library preparation and single-cell RNA-sequencing.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Jonathan Griffiths

PROVIDER: E-MTAB-7325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A single-cell molecular map of mouse gastrulation and early organogenesis.

Pijuan-Sala Blanca B Griffiths Jonathan A JA Guibentif Carolina C Hiscock Tom W TW Jawaid Wajid W Calero-Nieto Fernando J FJ Mulas Carla C Ibarra-Soria Ximena X Tyser Richard C V RCV Ho Debbie Lee Lian DLL Reik Wolf W Srinivas Shankar S Simons Benjamin D BD Nichols Jennifer J Marioni John C JC Göttgens Berthold B

Nature 20190220 7745

Across the animal kingdom, gastrulation represents a key developmental event during which embryonic pluripotent cells diversify into lineage-specific precursors that will generate the adult organism. Here we report the transcriptional profiles of 116,312 single cells from mouse embryos collected at nine sequential time points ranging from 6.5 to 8.5 days post-fertilization. We construct a molecular map of cellular differentiation from pluripotency towards all major embryonic lineages, and explor ...[more]

PMID: 30787436

Similar Datasets

Single-cell RNAseq of Tal1 knockout chimeric mouse embryos at embryonic days 7.5 and 8.5 of mouse development.

Project description:Chimeric embryos were generated to investigate the effect of Tal1 knockout in mouse embryos by single-cell RNA-sequencing. Tal1 is an essential transcription factor for the formation of the embryonic blood. Embryo chimerism permits the analysis of the effects of Tal1 knockout without the confounding effects of the absence of embryonic blood, which results in global developmental failures. Embryos were generated by blastocyst injection of tdTomato-labelled, Tal1-/- mouse embryonic stem cells into wild type embryos. After blastocyst harvest, cells were flow-sorted before 10X Genomics library preparation and single-cell RNA-sequencing.

2018-12-05 | E-MTAB-6968 | biostudies-arrayexpress

Single-cell RNAseq of control chimeric mouse embryos at embryonic days 7.5 and 8.5 of mouse development

Project description:Chimeric mouse embryos were generated as a control experiment to understand the contribution of host vs. injected cells to the developing embryo. Embryos were generated by blastocyst injection of tdTomato-labelled mouse embryonic stem cells into wild type embryos. After blastocyst harvest, cells were flow-sorted before 10X Genomics library preparation and single-cell RNA-sequencing.

2018-12-05 | E-MTAB-7324 | biostudies-arrayexpress

Single-cell RNAseq of additional control chimeric mouse embryos at embryonic day 8.5 of mouse development

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2020-08-31 | E-MTAB-8812 | biostudies-arrayexpress

Single-cell RNAseq of Brachyury knockout chimeric mouse embryos at embryonic days 8.5 and 7.5 of mouse development.

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Single-cell RNAseq of Mixl1 knockout chimeric mouse embryos at embryonic day 8.5 of mouse development.

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NPKLH data

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2020-05-12 | E-MTAB-8904 | biostudies-arrayexpress

Single-cell gene expression of germinal center B cells from CXCL12-conditionally deficient and –sufficient mice

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2020-05-12 | E-MTAB-8454 | biostudies-arrayexpress

Single-cell gene expression of lymph node B cell-interacting reticular cells

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2020-05-12 | E-MTAB-8445 | biostudies-arrayexpress

Identification of cell surface markers and establishment of monolayer differentiation protocol for retinal pigment epithelial cells.

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2020-02-29 | E-MTAB-7742 | biostudies-arrayexpress

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