Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Study on miRNA expression pattern in Aging Mouse Hippocampal Neurons

ABSTRACT: Mouse hippocampal microRNA 3 samples

ORGANISM(S): Mus musculus

SUBMITTER: keetae kim

PROVIDER: E-GEOD-74796 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

miR-204 downregulates EphB2 in aging mouse hippocampal neurons.

Mohammed Chand Parvez Danka CP Rhee Hwanseok H Phee Bong-Kwan BK Kim Kunhyung K Kim Hee-Jin HJ Lee Hyehyeon H Park Jung Hoon JH Jung Jung Hee JH Kim Jeong Yeon JY Kim Hyoung-Chin HC Park Sang Ki SK Nam Hong Gil HG Kim Keetae K

Aging cell 20160122 2

Hippocampal synaptic function and plasticity deteriorate with age, often resulting in learning and memory deficits. As MicroRNAs (miRNAs) are important regulators of neuronal protein expression, we examined whether miRNAs may contribute to this age-associated decline in hippocampal function. We first compared the small RNA transcriptome of hippocampal tissues from young and old mice. Among 269 hippocampal miRNAs, 80 were differentially expressed (≥ twofold) among the age groups. We focused on 36 ...[more]

PMID: 26799631

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Project description:Calorie restriction (CR) enhances longevity and mitigates aging phenotypes in numerous species. Physiological responses to CR are cell-type specific and variable throughout the lifespan; however, the mosaic of molecular changes responsible CR benefits remain unclear, particularly in brain regions susceptible to deterioration throughout aging. Thus, we examined the influence of long-term CR on the CA1 hippocampal region, a key learning and memory brain area that is vulnerable to age-related pathologies, such as Alzheimerâs disease (AD). Through mRNA sequencing and NanoString nCounter analysis, we demonstrate that one year of CR feeding suppresses an age-dependent signature of 882 genes functionally associated with synaptic transmission-related pathways, including calcium signaling, long-term potentiation (LTP), and Creb signaling in wild-type mice. By comparing the influence of CR on hippocampal CA1 region transcriptional profiles at younger- (5 months) and older-adult (15 months) timepoints, we identify conserved upregulation of proteome quality control and calcium buffering genes, including heat shock 70 kDa proteins 1b and 5 (Hspa1b and Hspa5), protein disulfide isomerase family A members 4 and 6 (Pdia4 and Pdia6), and calreticulin (Calr). Expression levels of putative neuroprotective factors, klotho (Kl) and transthyretin (Ttr), are also elevated by CR throughout adulthood, although the global CR-specific expression profiles at young and older timepoints are highly divergent. At a previously unachieved resolution, our results demonstrate conserved activation of neuroprotective gene signatures and broad CR-suppression of age-dependent hippocampal CA1 region expression changes, indicating that CR functionally maintains a more youthful transcriptional state within hippocampal CA1 throughout aging. Hippocampal CA1 region mRNA profiles of younger- (5 months) and older-adult (15 months) mice on calorie-restricted (CR) and normal (AD) diets were generated by deep sequencing using Illumina HiSeq 2500.

Dataset Information

Study on miRNA expression pattern in Aging Mouse Hippocampal Neurons

Publications

miR-204 downregulates EphB2 in aging mouse hippocampal neurons.

Similar Datasets

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