Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of rat lung alveolar development


ABSTRACT: Lung alveolarization is a complex process that involves interactions between several cell types and leads to considerable increase in gas-exchange surface area. The step designated secondary septation includes elastogenesis from interstitial fibroblasts. We used microarrays to detail the global programme of fibroblast gene expression that underly secondary septation, and identified numerous genes that undergo signficant changes in expression Experiment Overall Design: Rat lung fibroblats were extemporaneously isolated at successive stages of postnatal development that flank the septation period for RNA extraction and hybridization on Affymetrix microarrays. Three biological replicates from three different litters were prepared for each time point.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Jacques Bourbon 

PROVIDER: E-GEOD-7491 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene expression profiling in lung fibroblasts reveals new players in alveolarization.

Boucherat Olivier O   Franco-Montoya Marie-Laure ML   Thibault Christelle C   Incitti Roberto R   Chailley-Heu Bernadette B   Delacourt Christophe C   Bourbon Jacques R JR  

Physiological genomics 20071002 1


Little is known about the molecular basis of lung alveolarization. We used a microarray profiling strategy to identify novel genes that may regulate the secondary septation process. Rat lung fibroblasts were extemporaneously isolated on postnatal days 2, 7, and 21, i.e., before, during, and after septation, respectively. Total RNA was extracted, and cRNAs were hybridized to Affymetrix rat genome 230 2.0 microarrays. Expression levels of a selection of genes were confirmed by real-time PCR. In ad  ...[more]

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