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Transcription profiling of primary human macrophages treated with TGF-beta

ABSTRACT: The goal of the study was to identify the effects of TGF-beta on primary human macrophages maturated under different conditions. Experiment Overall Design: For the analysis of TGF-beta effects macrophages were differentiated in the presence of IL-4 or IL-4 in combination with dexamethasone. After 5 days differentiation mature macrophages were stimulated by TGF-beta for 24 hours. In the case of IL-4/dexamethasone stimulation an additional data point was taken. In this case macrophages were also stimulated by TGF-beta for 3 hours. For every group 5 independent donors were taken.

ORGANISM(S): Homo sapiens

SUBMITTER: Alexei Gratchev

PROVIDER: E-GEOD-7568 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Activation of a TGF-beta-specific multistep gene expression program in mature macrophages requires glucocorticoid-mediated surface expression of TGF-beta receptor II.

Gratchev Alexei A Kzhyshkowska Julia J Kannookadan Sheila S Ochsenreiter Miriam M Popova Anna A Yu Xiaolei X Mamidi Srinivas S Stonehouse-Usselmann Eugenia E Muller-Molinet Isabelle I Gooi Liming L Goerdt Sergij S

Journal of immunology (Baltimore, Md. : 1950) 20080501 10

Alternatively activated (M2) macrophages regulate steady state-, cancer-, and inflammation-related tissue remodeling. They are induced by Th2-cytokines and glucocorticoids (GC). The responsiveness of mature macrophages to TGF-beta, a cytokine involved in inflammation, cancer, and atherosclerosis, is currently controversial. Recently, we demonstrated that IL-17 receptor B is up-regulated in human monocyte-derived macrophages differentiated in the presence of Th2 cytokines IL-4 and TGF-beta1. In t ...[more]

PMID: 18453574

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