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Unknown,Transcriptomics,Genomics,Proteomics

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Image based identification and targeting of cancer stem cells in pancreatic adenocarcinoma (PDAC)

ABSTRACT: Purpose: The goals of this study were to identify quantitative gene expression differences between wild type, Musashi1 null, Msuashi2 null and Musashi1/Musashi2 null MIAPaCa2 pancreatic cancer cells mRNA profiles of MIA PaCa-2 cancer cells were generated by deep sequencing, in triplicate, using Illumina HiSeq2500.

ORGANISM(S): Homo sapiens

SUBMITTER: Roman Sasik

PROVIDER: E-GEOD-75797 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Image-based detection and targeting of therapy resistance in pancreatic adenocarcinoma.

Fox Raymond G RG Lytle Nikki K NK Jaquish Dawn V DV Park Frederick D FD Ito Takahiro T Bajaj Jeevisha J Koechlein Claire S CS Zimdahl Bryan B Yano Masato M Kopp Janel J Kritzik Marcie M Sicklick Jason J Sander Maike M Grandgenett Paul M PM Hollingsworth Michael A MA Shibata Shinsuke S Pizzo Donald D Valasek Mark M Sasik Roman R Scadeng Miriam M Okano Hideyuki H Kim Youngsoo Y MacLeod A Robert AR Lowy Andrew M AM Reya Tannishtha T

Nature 20160606 7607

Pancreatic intraepithelial neoplasia is a pre-malignant lesion that can progress to pancreatic ductal adenocarcinoma, a highly lethal malignancy marked by its late stage at clinical presentation and profound drug resistance. The genomic alterations that commonly occur in pancreatic cancer include activation of KRAS2 and inactivation of p53 and SMAD4 (refs 2-4). So far, however, it has been challenging to target these pathways therapeutically; thus the search for other key mediators of pancreatic ...[more]

PMID: 27281208

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Image based identification and targeting of cancer stem cells in pancreatic adenocarcinoma (PDAC)

Project description:Purpose: The goals of this study were to identify quantitative gene expression differences between wild type, Musashi1 null, Msuashi2 null and Musashi1/Musashi2 null MIAPaCa2 pancreatic cancer cells

2016-06-15 | GSE75797 | GEO

DNA-damage induced differentiation of leukemic cells as an anti-cancer barrier

Project description:Here we show that the histone methyltransferase MLL4 (Kmt2d) is required for stem cell activity and an aggressive form of acute myeloid leukemia (AML) harboring the MLL-AF9 oncogene. MLL4 exerts its function by regulating transcriptional programs associated with the anti-oxidant response. The role of Mll4 (Kmt2d) in regulating the transcriptome of primary and transformed hematopoietic stem cells was studied.

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Identification of tomato proteins that interact with Replication initiator protein (Rep) of the geminivirus TYLCV

Project description:In this study, an GFP-based affinity purification followed by mass spectrometry (AP-MS) approach is used in order to identify new tomato proteins that interact with the Replication initiator protein (Rep, AL1, C1) from Tomato yellow leaf curl virus (TYLCV).

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MicroRNA screening in human pancreatic carcinoma cell lines

Project description:No reliable predictors of susceptibility to gemcitabine chemotherapy exist in pancreatic ductal adenocarcinoma. MicroRNAs (miR) are epigenetic gene regulators with tumorsuppressive or oncogenic roles in various carcinomas. This study assesses chemoresistant PDAC for its specific miR expression pattern. Gemcitabine-resistant variants of two mutant p53 human pancreatic adenocarcinoma cell lines were established. MicroRNA screening was investigated by microarray. Gemcitabine-resistant PANC-1 (PANC-1-GR) and MIA-PaCa-2 (MIA-PaCa-2-GR) cell clones were produced by exposing the parental cells to repeated pulsatile gemcitabine treatment over 3 days with constant sublethal concentrations followed by recovery-periods with agent-free medium until the cells recovered exponentially. Parental PANC-1 cells were treated with 0.4ÂµM gemcitabine cycles for approximately 9 months. Parental MIA-PaCa-2 cells were exposed to 0.06ÂµM gemcitabine cycles for approximately 12 months. Affymetrix GeneChip miRNA microarrays (Affymetrix UK Ltd., High Wycombe, UK) were performed in parental and chemoresistant PANC-1 and MIA-PaCa-2 cells after 29 chemotherapy cycles using the manufacturersÂ´ protocols. The samples were prepared from 1Âµg of total-RNA in accordance with the Affymetrix FlashTag Biotin HSR RNA Labeling Kit. The targets were hybridized overnight to Affymetrix GeneChip miRNA arrays. Following hybridization, the arrays were washed and stained using the Affymetrix GeneChip Fluidics Station 450 and scanned using the Affymetrix GeneChip Scanner 3000 7G. Microarray data quality was checked as recommended by the manufacturer and by the quality metrics in the Partek Genomics Suite software (Partek Inc., St. Louis, MO).

2015-11-03 | E-GEOD-74565 | biostudies-arrayexpress

microRNA screening in human pancreatic adenocarcinoma

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-11-03 | E-GEOD-74574 | biostudies-arrayexpress

Transcription profiling of Capsicum fruit ripening process

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Kinase activities in pancreatic ductal adenocarcinoma with prognostic and therapeutic avenues

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Plastoglobules are a biosynthetic platform contributing to carotenoid accumulation during tomato fruit maturation

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2023-03-11 | PXD035763 | Pride

Characterization of the tomato fruit pericarp proteome (2)

Project description:In this work, we present an extended characterization of the proteome of the tomato pericarp in its ripe red stage. An extensive fractionation of peptides through high pH reverse phase was performed associated to LC-MS/MS analysis on a Thermofisher scientific Q-Exactive

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Characterization of the tomato fruit pericarp proteome

2016-12-22 | PXD004932 | Pride