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Unknown,Transcriptomics,Genomics,Proteomics

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M1A marks translation initiation sites in human and mouse messenger RNA [microarray]

ABSTRACT: We developed a novel approach, m1A-seq, for high-resolution mapping of the transcriptome-wide m1A landscape, based on antibody-mediated capture followed by massively parallel sequencing. Using this method we performed immunodepletion of methylated transcipts to assess the average methylation level Immunodepletion of m1A modified transcripts in human compared to the input, allows for estimation of the fraction of methylated transcripts. We compared the expression levels of sup (immunodepleted) and input methylated transcripts to deduce the fraction of m1A methylation.

ORGANISM(S): Homo sapiens

SUBMITTER: Sharon Moshitch-Moshkovitz

PROVIDER: E-GEOD-76057 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA.

Dominissini Dan D Nachtergaele Sigrid S Moshitch-Moshkovitz Sharon S Peer Eyal E Kol Nitzan N Ben-Haim Moshe Shay MS Dai Qing Q Di Segni Ayelet A Salmon-Divon Mali M Clark Wesley C WC Zheng Guanqun G Pan Tao T Solomon Oz O Eyal Eran E Hershkovitz Vera V Han Dali D Doré Louis C LC Amariglio Ninette N Rechavi Gideon G He Chuan C

Nature 20160210 7591

Gene expression can be regulated post-transcriptionally through dynamic and reversible RNA modifications. A recent noteworthy example is N(6)-methyladenosine (m(6)A), which affects messenger RNA (mRNA) localization, stability, translation and splicing. Here we report on a new mRNA modification, N(1)-methyladenosine (m(1)A), that occurs on thousands of different gene transcripts in eukaryotic cells, from yeast to mammals, at an estimated average transcript stoichiometry of 20% in humans. Employin ...[more]

PMID: 26863196

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