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Transcription profiling of Drosophila head from y w strain - timepoints control strain

ABSTRACT: 6 Timepoint microarray from control strain Experiment Overall Design: Fly head microarray from 6 Timepoints using yw strain

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sebastian Kadener

PROVIDER: E-GEOD-7652 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component.

Kadener Sebastian S Stoleru Dan D McDonald Michael M Nawathean Pipat P Rosbash Michael M

Genes & development 20070619 13

Many organisms use circadian clocks to keep temporal order and anticipate daily environmental changes. In Drosophila, the master clock gene Clock promotes the transcription of several key target genes. Two of these gene products, PER and TIM, repress CLK-CYC-mediated transcription. To recognize additional direct CLK target genes, we designed a genome-wide approach and identified clockwork orange (cwo) as a new core clock component. cwo encodes a transcriptional repressor that synergizes with PER ...[more]

PMID: 17578907

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Project description:6 Timepoint microarray from control strain Keywords: Timepoints yw

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Nascent-seq indicates widespread cotranscriptional RNA editing in Drosophila

Project description:The RNA editing enzyme ADAR chemically modifies adenosine (A) to inosine (I), which is interpreted by the ribosome as a guanosine. Here we assess cotranscriptional A-to-I editing in Drosophila, by isolating nascent RNA from adult fly heads and subjecting samples to high-throughput sequencing. There are a large number of edited sites within nascent exons. Nascent RNA from an ADAR null mutant strain was also sequenced, indicating that almost all A-to-I events require ADAR. Moreover, mRNA editing levels correlate with editing levels within the cognate nascent RNA sequence, indicating that the extent of editing is set cotranscriptionally. Surprisingly, the nascent data also identify an excess of intronic over exonic editing sites. These intronic sites occur preferentially within introns that are poorly spliced cotranscriptionally, suggesting a link between editing and splicing. We conclude that ADAR-mediated editing is more widespread than previously indicated and largely occurs cotranscriptionally. GSM914095: Fly genomic DNA sequencing. Sequenced on the Illumina GA II. GSM914102-GSM914113: Fly head nascent RNA profiles over 6 time points of a 12hr light:dark cycle in duplicate; sequenced on the Illumina GA II. GSM914114-GSM914119: Fly head nascent RNA profiles of yw, FM7, ADAR0 males in duplicate; sequenced on the HiSeq2000. GSM915213-GSM915214: Fly head mRNA profiles over 2 time points of a 12hr light:dark cycle; sequenced on the Illumina GA II. GSM915215-GSM915220: Fly head mRNA profiles over 6 time points of a 12hr light:dark cycle; paired-end sequenced on the Illumina GA II. GSM915221-GSM91526: Fly head mRNA profiles over 6 time points of a 12hr light:dark cycle; sequenced on the Illumina GA II.

2012-05-31 | E-GEOD-37232 | biostudies-arrayexpress

Aging in heads from adult Drosophila males, w118 strain

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Chronic potassium chloride (KCl) treatment induced gene expression changes in Drosophila head

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Effect of vigabatrin on gene expression in Drosophila head

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Effect of gabapentin on gene expression in Drosophila head

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2007-10-05 | GSE7202 | GEO

Effect of sodium valproate on gene expression in Drosophila head

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