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Unknown,Transcriptomics,Genomics,Proteomics

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Neuronal and glial Gene expression from control and healthy patient iPSC-derived forebrain neurons (A)

ABSTRACT: Neuronal or glial cell fates related gene expression from single cells with variety of sAPPa or total AÎ² secretion profiles were presented here. Single living human iPSC-derived forebrain neuronal cells with variety of sAPPa or total AÎ² secretion profiles were selected and examined for gene expression.

ORGANISM(S): Homo sapiens

SUBMITTER: Tracy Young-Pearse

PROVIDER: E-GEOD-76545 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Neuronal and glial Gene expression from control and healthy patient iPSC-derived cortical neurons (B)

Project description:Neuronal or glial cell fates related gene expression from single cells with variety of sAPPa or total AÎ² secretion profiles were presented here. Single living human iPSC-derived forebrain neuronal cells with variety of sAPPa or total AÎ² secretion profiles were selected and examined for gene expression.

2016-01-06 | E-GEOD-76547 | biostudies-arrayexpress

Neuronal and glial Gene expression from control and healthy patient iPSC-derived cortical neurons

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2016-01-06 | E-GEOD-76551 | biostudies-arrayexpress

Design, characterization, and use of a novel amyloid Î²-protein control for assembly, neurotoxicity, and gene expression studies

Project description:A key pathogenic agent in Alzheimerâs disease (AD) is the amyloid Î²-protein (AÎ²), which self-assembles into a variety of neurotoxic structures. Establishing structure-activity relationships for these assemblies is critical for proper therapeutic target identification and design. We examined the effects of AÎ² monomers, dimers, higher-order oligomers, and fibrils on gene expression in primary rat hippocampal neurons. As opposed to âreverseâ AÎ² or non-AÎ² peptides typically used as controls in such studies, we designed novel scrambled AÎ² peptides predicted to behave distinctly from native AÎ². Significant changes in gene expression were observed for all peptide assemblies, but fibrils induced the largest changes. Significant changes in gene expression were observed for all peptide assemblies, but fibrils induced the largest changes. Weighted gene co-expression network analysis (WGCNA) revealed two predominant gene modules related to AÎ² treatment. Many genes within these modules were associated with inflammatory signaling pathways We examined the effects of AÎ² monomers, dimers, higher-order oligomers, and fibrils on gene expression in primary rat hippocampal neurons. Novel scrambled AÎ² peptides, as opposed to âreverseâ AÎ² or non-AÎ² peptides, were used as controls. Please note that the 'XL42_1' sample was excluded from data processing as an outlier (resulting total 23 sample records). However the 'XL42_1' raw data is provided in the 'non_normalized.txt' (total 24 data columns).

2016-08-24 | E-GEOD-85952 | biostudies-arrayexpress

Spatially resolved transcriptomic profiling: an experimental comparison of GeoMx Digital Spatial Profiling and Visium CytAssist spatial transcriptomics in three cancer types (GeoMx)

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MicroRNA expression changes in Bone Marrow Stromal Cells from Myeloma Patients vs Normal Patients, and From Normal Patients cultured with or without Myeloma cells in 3D culture

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Tuberculosis alters immune-metabolic pathways resulting in perturbed IL-1 responses

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Brain proteome analysis of APPswe mouse model at the early stages of Alzheimer's disease pathology

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