Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

48 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Neuronal and glial Gene expression from control and healthy patient iPSC-derived cortical neurons (B)

ABSTRACT: Neuronal or glial cell fates related gene expression from single cells with variety of sAPPa or total AÎ² secretion profiles were presented here. Single living human iPSC-derived forebrain neuronal cells with variety of sAPPa or total AÎ² secretion profiles were selected and examined for gene expression.

ORGANISM(S): unidentified

SUBMITTER: Tracy Young-Pearse

PROVIDER: E-GEOD-76547 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Neuronal and glial Gene expression from control and healthy patient iPSC-derived forebrain neurons (A)

Project description:Neuronal or glial cell fates related gene expression from single cells with variety of sAPPa or total AÎ² secretion profiles were presented here. Single living human iPSC-derived forebrain neuronal cells with variety of sAPPa or total AÎ² secretion profiles were selected and examined for gene expression.

2016-01-06 | E-GEOD-76545 | biostudies-arrayexpress

Neuronal and glial Gene expression from control and healthy patient iPSC-derived cortical neurons

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-01-06 | E-GEOD-76551 | biostudies-arrayexpress

Design, characterization, and use of a novel amyloid Î²-protein control for assembly, neurotoxicity, and gene expression studies

Project description:A key pathogenic agent in Alzheimerâs disease (AD) is the amyloid Î²-protein (AÎ²), which self-assembles into a variety of neurotoxic structures. Establishing structure-activity relationships for these assemblies is critical for proper therapeutic target identification and design. We examined the effects of AÎ² monomers, dimers, higher-order oligomers, and fibrils on gene expression in primary rat hippocampal neurons. As opposed to âreverseâ AÎ² or non-AÎ² peptides typically used as controls in such studies, we designed novel scrambled AÎ² peptides predicted to behave distinctly from native AÎ². Significant changes in gene expression were observed for all peptide assemblies, but fibrils induced the largest changes. Significant changes in gene expression were observed for all peptide assemblies, but fibrils induced the largest changes. Weighted gene co-expression network analysis (WGCNA) revealed two predominant gene modules related to AÎ² treatment. Many genes within these modules were associated with inflammatory signaling pathways We examined the effects of AÎ² monomers, dimers, higher-order oligomers, and fibrils on gene expression in primary rat hippocampal neurons. Novel scrambled AÎ² peptides, as opposed to âreverseâ AÎ² or non-AÎ² peptides, were used as controls. Please note that the 'XL42_1' sample was excluded from data processing as an outlier (resulting total 23 sample records). However the 'XL42_1' raw data is provided in the 'non_normalized.txt' (total 24 data columns).

2016-08-24 | E-GEOD-85952 | biostudies-arrayexpress

Neuronal and glial Gene expression from control and healthy patient iPSC-derived cortical neurons (B)

Project description:Neuronal or glial cell fates related gene expression from single cells with variety of sAPPa or total Aβ secretion profiles were presented here.

2016-01-06 | GSE76547 | GEO

Neuronal and glial Gene expression from control and healthy patient iPSC-derived forebrain neurons (A)

Project description:Neuronal or glial cell fates related gene expression from single cells with variety of sAPPa or total Aβ secretion profiles were presented here.

2016-01-06 | GSE76545 | GEO

Brain proteome analysis of APPswe mouse model at the early stages of Alzheimer's disease pathology

Project description:Plaques consisting of amyloid-Î² (AÎ²) in the brain are characteristic for patients with AlzheimerÂ´s disease. AÎ² is enzymatically generated of the amyloid precursor protein (APP). During the disease, AÎ² starts to aggregate forming soluble oligomers, soluble protofibrils and eventually insoluble fibrils. AÎ² pathology starts mainly at the hippocampus and the surrounding cortical area. Mice overexpressing the Aï�¢-precursor protein with the Swedish mutation (APPswe) are one of the most commonly used animal models in Alzheimerâ€™s field. These mice overexpress APP with the Swedish mutation (KM670/671NL), which is adjacent to the Î²-secretase cleavage site on APP. This results in increased production of AÎ². The aim of this study was to study the proteome of APPswe brain at the early stages (7-8 months old) of the disease, before plaque onset, which starts at around 11-12 months of age in this mouse model. Homogenates of the hippocampus and the rest of the cerebrum from 7-8 months-old APPswe and wild-type (WT) controls were lysed. Afterwards, filter aided tryptic digestion was performed. The resulting peptides were analyzed using LC-UDMSE. The data was searched against a randomized mouse database and label-free quantification analysis was done. In total 2487 proteins were found. In the hippocampus of APPswe mice, the levels of 1283 proteins were significantly altered compared to WT controls. While 1379 proteins were significantly altered in the rest of the cerebrum of APPswe mice compared to WT controls. Among these, mitochondrial proteins and proteins involved in neuron's development were significantly downregulated, suggesting mitochondrial dysfunction and dysregulation of neuronâ€™s growth in the brain of APPswe mice at already this stage of the disease.

2021-06-28 | PXD026566 | Pride

Expression Profile of in vitro Expanded Human Tregs

Project description:Understanding human Regulatory T cell (Treg) heterogeneity may identify markers of disease pathogenesis and facilitate the development of optimized cellular therapeutics. Previous analysis revealed that the co-inhibitory receptor T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain (TIGIT) was highly expressed on tTreg. The negative regulator TIGIT and the co-stimulatory factor CD226 bind the common ligand CD155. Human regulatory T cells (CD4+CD25+CD127-/lo) from adult peripheral blood were sub-fractionated based on the markers CD226 and TIGIT and were subsequently expanded in vitro according to clinical expansion protocols. The transcriptional profile of the final cell products uncovered considerable heterogeneity in terms of in vitro expansion and suppressive capacity. Most notably, the CD226+TIGIT- fraction adopted a transcriptional profile most similar to that of conventional T cells, including the capacity for effector cytokine production. Tregs and corresponding Tconv were expanded from peripheral blood of three normal healthy control male subjects.

2015-04-30 | E-GEOD-62015 | biostudies-arrayexpress

Pro-osteogenic trophic effects by PKA activation in human mesenchymal stromal cells

Project description:Human mesenchymal stromal cells (hMSCs) are able to differentiate into a wide variety of cell types, which makes them an interesting source for tissue engineering applications. On the other hand, these cells also secrete a broad panel of growth factors and cytokines that can exert trophic effects on surrounding tissues. In bone tissue engineering applications, the general assumption is that direct differentiation of hMSCs into osteoblasts accounts for newly observed bone formation in vivo. However, the secretion of bone-specific growth factors, but also pro-angiogenic factors, could also contribute to this process. We recently demonstrated that secretion of bone specific growth factors can be enhanced by treatment of hMSCs with the small molecule db-cAMP (cAMP) and here we investigate the biological activity of these secreted factors. We demonstrate that conditioned medium contains a variety of secreted growth factors, with differences between medium from basic-treated and cAMP-treated hMSCs. We show that conditioned medium from cAMP-treated hMSCs increases proliferation of various cell types and also induces osteogenic differentiation, whereas it has differential effects on migration. Microarray analysis on hMSCs exposed to conditioned medium confirmed upregulation of pathways involved in proliferation as well as osteogenic differentiation. Our data suggests that trophic factors secreted by hMSCs can be tuned for specific applications and that a good balance between differentiation on the one hand and secretion of bone trophic factors on the other, could potentially enhance bone formation for bone tissue engineering applications. For more information check: https://cbit.maastrichtuniversity.nl/

2018-10-06 | E-MTAB-7224 | biostudies-arrayexpress

Digital expression profiling highlights a distinct live RNA signature in high-risk Primary Biliary Cholangitis (PBC)

Project description:Using outcome after long term follow-up to define risk at disease presentation high risk (n=9 who went on to require liver transplantation) and low risk (n=7 who responded fully to UDCA) patients were identified and their first liver biopsies retrieved. RNA was successfully extracted and analysed using nanostringÂ® transcriptomics. Patients with progressive disease appear to have a distinct molecular signature. high risk (n=9 who went on to require liver transplantation) and low risk (n=7 who responded fully to UDCA) patient material was processed along with non-diseased control liver (n=8)

2016-04-02 | E-GEOD-79850 | biostudies-arrayexpress

Profiling transcriptional and translational response to anoxia or hypoxia of genes in DNA damage response and repair pathways in human fibroblast and prostate cancer cells using NanoString nCounter.

Project description:Identification of genes in DNA damage response and repair pathways differentially transcribed or translated under anoxia or hypoxia in GM05757 normal human fibroblast cells and DU145 human prostate cancer cells. Comparison of mRNA abundance and translation efficiency of genes in DNA damage response and repair pathways in selected anoxia/hypoxia-treated cells with those in normoxia-treated controls.

2015-07-01 | E-GEOD-52461 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data