Ontology highlight
ABSTRACT:
ORGANISM(S): Escherichia coli
SUBMITTER: Chrystala Constantinidou
PROVIDER: E-GEOD-7695 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Journal of bacteriology 20071005 24
The use of whole-genome microarrays for monitoring mutagenized or otherwise engineered genetic derivatives is a potentially powerful tool for checking genomic integrity. Using comparative genomic hybridization of a number of unrelated, directed deletion mutants in Escherichia coli K-12 MG1655, we identified unintended secondary genomic deletions in the flhDC region in delta fnr, delta crp, and delta creB mutants. These deletions were confirmed by PCR and phenotypic tests. Our findings show that ...[more]