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MG1655 araC arabinose transcription profiling

ABSTRACT: AraC is an Escherichia coli transcription factor that regulates genes in response to the presence/absence of arabinose. We used transcription profiling to determine RNA levels in Escherichia coli K-12 strain MG1655 and MG1655 delta araC grown either in the absence or the presence of arabinose. Thus, we identified known and novel AraC-regulated genes. Data presented here are raw .CEL files as well as fully analysed data using GeneSpring

ORGANISM(S): Escherichia coli K-12

SUBMITTER: Joseph Wade

PROVIDER: E-MTAB-1916 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-scale analyses of Escherichia coli and Salmonella enterica AraC reveal noncanonical targets and an expanded core regulon.

Stringer Anne M AM Currenti Salvatore S Bonocora Richard P RP Baranowski Catherine C Petrone Brianna L BL Palumbo Michael J MJ Reilly Andrew A AA Zhang Zhen Z Erill Ivan I Wade Joseph T JT

Journal of bacteriology 20131122 3

Escherichia coli AraC is a well-described transcription activator of genes involved in arabinose metabolism. Using complementary genomic approaches, chromatin immunoprecipitation (ChIP)-chip, and transcription profiling, we identify direct regulatory targets of AraC, including five novel target genes: ytfQ, ydeN, ydeM, ygeA, and polB. Strikingly, only ytfQ has an established connection to arabinose metabolism, suggesting that AraC has a broader function than previously described. We demonstrate ...[more]

PMID: 24272778

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