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MRNA-seq of nuclear RNA extracted from T4 and T5 neurons of D. melanogaster

ABSTRACT: T4 and T5 neurons are components of the neuronal circuit for motion vision in flies. To identify genes involved in neuronal computation of T4 and T5 neurons, we perfomed transcriptome analysis. Nuclei of T4 and T5 neurons were immunoprecipitated, total RNA was harvested and used for mRNA-seq with Illumina technology. In two biological replicates, we mapped 154 and 119 million reads to D. melanogaster genome. mRNA-seq provided information about expression levels of 17,468 annotated transcripts in the T4 and T5 neurons. Cell type – specific transcriptome analysis of the RNA isolated from immunoprecipitated nuclei, performed in two biological replicates

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Katarina Pankova

PROVIDER: E-GEOD-77198 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-Seq Transcriptome Analysis of Direction-Selective T4/T5 Neurons in Drosophila.

Pankova Katarina K Borst Alexander A

PloS one 20160929 9

Neuronal computation underlying detection of visual motion has been studied for more than a half-century. In Drosophila, direction-selective T4/T5 neurons show supralinear signal amplification in response to stimuli moving in their preferred direction, in agreement with the prediction made by the Hassenstein-Reichardt detector. Nevertheless, the molecular mechanism explaining how the Hassenstein-Reichardt model is implemented in T4/T5 cells has not been identified yet. In the present study, we u ...[more]

PMID: 27684367

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