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Gene expression profiles of P1 ureter isolated from SMGA (Actg2) transgenic mice. (GUDMAP Series ID: 14)

ABSTRACT: The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital tract. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of postnatal day 1 ureters. Keywords: Gene expression analysis of the postnatal day 1 mouse ureter

ORGANISM(S): Mus musculus

PROVIDER: GSE6963 | GEO | 2007/02/08

SECONDARY ACCESSION(S): PRJNA99325

REPOSITORIES: GEO

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Gene expression profiles of adult ureter isolated from SMGA (Actg2) transgenic mice. (GUDMAP Series ID: 15)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the adult ureter. Keywords: Gene expression analysis of adult mouse ureter

2007-05-25 | GSE7887 | GEO

Transcription profiling of mouse postnatal day 1 ureter (GUDMAP Series_id: 14)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital tract. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of postnatal day 1 ureters. Experiment Overall Design: Ureters were isolated from postnatal day 1 SMGA/EGFP transgenic mice and RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

2008-06-15 | E-GEOD-6963 | biostudies-arrayexpress

Gene expression profiles of detrusor, stroma and urothelium isolated from SMGA (Actg2) transgenic neonatal bladders using LCM. (GUDMAP Series ID: 20)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of compartmental bladder tissues collected through laser capture microscopy. Keywords: Gene expression comparison from developing regions of the mouse postnatal day 1 and postnatal day 2 urogenital system.

2008-04-22 | GSE11221 | GEO

Gene expression profiles of P1 and adult bladders isolated from SMGA (Actg2) transgenic mice. (GUDMAP Series ID: 11)

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Transcription profiling of mouse SMGA/EGFP adult ureter. (GUDMAP Series_id: 15)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the adult ureter. Experiment Overall Design: Ureter regions from adult SMGA/EGFP animals were microdissected and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

2008-06-15 | E-GEOD-7887 | biostudies-arrayexpress

Gene expression profiles of E14 bladder, urogenital sinus and urethra isolated from SMGA (Actg2) transgenic mice. (GUDMAP Series ID: 6)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 bladder, urogenital sinus, and urethra. Keywords: Gene expression comparison from developing regions of the mouse embryonic day 14 urogenital system

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Gene expression profiles of E15 ureter (GUDMAP Series ID: 26)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing genitourinary tract. The central thesis is straightforward. The combination of microdissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here delineates the gene expression profiles of the e15 mouse ureter. Comparisons with the same compartments in the neonate will be made to determine changes in gene expression from relatively undifferentiated tissue (e15) to a highly differentiated structure (neonate). FVB/N mice were time mated. At embryonic day 15 mice were euthanized by decapitation and the ureters were microdissected, cut from pelvis to the bladder insertion point and snap frozen in liquid nitrogen. Total RNA was isolated for gene expression analysis using the Affymetrix GeneChip Mouse Genome 430 2.0 Array.

2009-04-25 | E-GEOD-15795 | biostudies-arrayexpress

Gene expression profiles of E15 ureter. (GUDMAP Series ID: 26)

2009-04-25 | GSE15795 | GEO

Transcription profiling of mouse postnatal day 1 and adult bladders

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 bladder, urogenital sinus, and urethra. Experiment Overall Design: Bladders, from postnatal day 1 and adult SMGA/EGFP animals were isolated by dissection and total RNA extracted for gene expression analysis using the Affymetrix MOE430 microarray chip.

2008-06-14 | E-GEOD-6466 | biostudies-arrayexpress

Gene expression profiles of E13 bladder compartments. (GUDMAP Series ID: 24)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here delineates the gene expression profiles of the mesenchymal and epithelial compartments of the e13 mouse bladder. Keywords: Mouse embryonic day 13 bladder mesenchyme and epithelium. FVB/N mice were time mated. At embryonic day 13 mice were euthanized by decapitation and the bladders were microdissected, cut just above the ureters, and treated with 20 µM EDTA for 20 minutes. Mesenchymal and epithelial compartments were then separated by rimming the bladder with a needle and harvested in RLT. Total RNA was isolated for gene expression analysis using the Affymetrix MOE430 microarray chip

2009-01-16 | E-GEOD-12618 | biostudies-arrayexpress

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