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Yeast cells: Mutant M1 vs. the control with and without oxidative stress

ABSTRACT: Transcription profiles of mutant M1 and the control in the absence/presence of 4mM H2O2 M1 is a mutated Saccharomyces cerevisiae strain. It was obtained through transcriptional engineering of general transcription factor TFIIB. Encoded by SUA7, TFIIB is reported to regulate the expression of over 730 genes in Saccharomyces cerevisiae. M1 showed significant growth improvement compared with the control when challenged with 4mM H2O2. It also had resistance towards high osmotic pressure (1.5M NaCl). Two-condition experiment (no stress and 4mM H2O2 stress): M1 vs the control. Two biological replicates for M1 and the control under each condition.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Hefang Geng

PROVIDER: E-GEOD-79037 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Yeast cells: Mutant M1 vs. the control with and without oxidative stress

Project description:Transcription profiles of mutant M1 and the control in the absence/presence of 4mM H2O2 M1 is a mutated Saccharomyces cerevisiae strain. It was obtained through transcriptional engineering of general transcription factor TFIIB. Encoded by SUA7, TFIIB is reported to regulate the expression of over 730 genes in Saccharomyces cerevisiae. M1 showed significant growth improvement compared with the control when challenged with 4mM H2O2. It also had resistance towards high osmotic pressure (1.5M NaCl).

2016-03-10 | GSE79037 | GEO

Expression analysis with Phospholipase C over expression

Project description:The regulation of phospholipid biosynthesis in Saccharomyces cerevisiae through cis-acting upstream activating sequence inositol (UASino) and trans-acting elements, such as the INO2-INO4 complex and OPI1 by inositol supplementation in growth is thoroughly studied. In this study, we provide evidence for the regulation of lipid biosynthesis by phosphatidylinositol-specific phospholipase C (PLC) through UASino and two trans-acting elements. Gene expression analysis and radiolabelling experiments demonstrated that the overexpression of rice PLC in yeast cells altered phospholipid biosynthesis at the levels of transcriptional and enzyme activity. There are two biological replicates two hour and five hour induction with galactose. The genome wide expression of Saccharomyces cerevisiae strain BY4741 harbouring rPLC-pYES2 and a vectro control (pYES2) were compare with each other. (Agilent Gene Expression Saccharomyces cerevisiae 8x15k array AMADID: 016333)

2012-04-23 | E-GEOD-35677 | biostudies-arrayexpress

Expression analysis with Phospholipase C over expression

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2012-09-01 | E-GEOD-35745 | biostudies-arrayexpress

Functional link between TFIIB and Mediator

Project description:In this study, we generate genomic maps of Mediator, Pol II, TBP, TFIIH, TFIIA, TFIIB, TFIIE, TFIIF, by ChIP coupled to next generation sequencing technology (ChIP-seq), in wild type strains from Saccharomyces cerevisiae and in a mutant for the Mediator essential subunit Med10

2017-01-24 | E-MTAB-4607 | biostudies-arrayexpress

Saccharomyces cerevisiae BY4741

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Oxidative quality control regulates response to ER stress through translation control

Project description:The transcriptomic response after 3 hours of DTT treatment shows there is not a drastic difference in most genes between WT and skn7â, yap1â . Vma13â, sse2â were also used as part of the study. Organism : Saccharomyces cerevisiae, Agilent Yeast Gene expression 8x15K Array AMADID: 016322

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Gene Expression Analysis in Different Macrophage Phenotypes

Project description:Macrophage activation is the main immunological process occurring during the development of several diseases, and the heterogeneity of macrophage activation or differentiation has been suggested to be involved in disease progression. In the present study, we attempted to identify molecules specifically expressed on human classically activated macrophages (M1) to investigate the significance of the M1-like phenotype in human diseases. Human monocyte-derived macrophages were differentiated into M1, M2a, M2b, and M2c phenotypes, and gene expression profiles were analyzed by cDNA microarray analysis and were used for bioinformatics examination. The gene expression profiles of murine macrophages were additionally evaluated. We identified guanylate-binding protein 5 (GBP5), which is associated with leucine-rich repeat protein 3-mediated inflammasome assembly in the M1 macrophages of both humans and mice. Notably, GBP5 protein expression was detected in cultured M1 macrophages by Western blot analysis. GBP5 is a useful candidate marker of the M1 phenotype. CD14+ monocytes from human PBMC were cultured with GM-CSF(10 ng mLâ1) or M-CSF (50 ng mLâ1) for seven days to differentiate into macrophages.To induce macrophage subtypes [M1, M1(-), M2a, M2b, and M2c], the macrophages were further stimulated for 24 h with LPS (10 ng mLâ1) + IFN-Î³ (50 ng mLâ1), IFN-Î³ (50 ng mLâ1), IL-4 (10 ng mLâ1), IL-1Î² (10 ng mLâ1), and IL-10 (10 ng mLâ1). Control macrophages (M0) were prepared by incubating for 24 h without additional factors.Two independent experiments were performed using different donors.

2016-08-10 | E-GEOD-85346 | biostudies-arrayexpress

Function of chemokine (CXC motif) ligand 12 in periodontal ligament fibroblasts

Project description:The periodontal ligament (PDL) is one of the connective tissues located between the tooth and bone. It is characterized by rapid turnover. Periodontal ligament fibroblasts (PDLFs) play major roles in the rapid turnover of the PDL. Microarray analysis of human PDLFs (HPDLFs) and human dermal fibroblasts (HDFs) revealed markedly high expression of chemokine (CXC motif) ligand 12 (CXCL12) in the HPDLFs, which plays an important role in the migration of mesenchymal stem cells (MSCs). The function of CXCL12 in the periodontal ligament was investigated in HPDLFs. CXCL12 in HPDLFs and HDFs was examined by microarray, RT-PCR, qRT-PCR and ELISA. It was also immunohistochemically examined in the PDL in vivo. Chemotactic ability of CXCL12 was evaluated both in PDLFs and HDFs with migration assay of MSCs. The expression of CXCL12 in the HPDLFs was much higher than that in HDFs in vitro. CXCL12 was localized in fibroblasts and extracellular matrix in the PDL in rats. Migration assay demonstrated that the number of migrated MSCs by HPDLFs was significantly higher than that by HDFs. In addition, the migrated MSCs also expressed CXCL12 and several genes that are familiar to fibroblasts. The results suggested that PDLFs are able to synthesize and secrete CXCL12 protein, and that CXCL12 induces migration of MSCs in the PDL in order to maintain rapid turnover of the PDL. The objective of this study was to investigate the function of CXCL12 in the PDL with rapid turnover.Microarray analysis was performed using a Whole Human Genome 8x60K (Agilent Technologies, Tokyo, Japan) containing approximately 44,000 transcripts. According to the manufacturerM-bM-^@M-^Ys protocol, total RNAs from HPDLFs and HDFs were labeled with Cy3 and hybridized on the microarray. The hybridization data for HPDLFs were compared with data for HDFs.

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Chlorella ingestion and suppression of resistin gene expression in borderline diabetics: a randomized, placebo-controlled study

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