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Functional link between TFIIB and Mediator

ABSTRACT: In this study, we generate genomic maps of Mediator, Pol II, TBP, TFIIH, TFIIA, TFIIB, TFIIE, TFIIF, by ChIP coupled to next generation sequencing technology (ChIP-seq), in wild type strains from Saccharomyces cerevisiae and in a mutant for the Mediator essential subunit Med10

INSTRUMENT(S): Illumina Genome Analyzer IIx, NextSeq 500, Illumina HiSeq 2000

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Werner Michel

PROVIDER: E-MTAB-4607 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture.

Eychenne Thomas T Novikova Elizaveta E Barrault Marie-Bénédicte MB Alibert Olivier O Boschiero Claire C Peixeiro Nuno N Cornu David D Redeker Virginie V Kuras Laurent L Nicolas Pierre P Werner Michel M Soutourina Julie J

Genes & development 20160929 18

Mediator is a large coregulator complex conserved from yeast to humans and involved in many human diseases, including cancers. Together with general transcription factors, it stimulates preinitiation complex (PIC) formation and activates RNA polymerase II (Pol II) transcription. In this study, we analyzed how Mediator acts in PIC assembly using in vivo, in vitro, and in silico approaches. We revealed an essential function of the Mediator middle module exerted through its Med10 subunit, implicati ...[more]

PMID: 27688401

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